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Cetuximab sensitivi...
Cetuximab sensitivity of head and neck squamous cell carcinoma xenografts is associated with treatment-induced reduction in EGFR, pEGFR, and pSrc.
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- Jedlinski, Adam, 1978- (författare)
- Linköpings universitet,Avdelning för neurobiologi,Medicinska fakulteten,Region Östergötland, Öron- näsa- och halskliniken US
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- Garvin, Stina, 1977- (författare)
- Linköpings universitet,Avdelning för neurobiologi,Medicinska fakulteten,Region Östergötland, Klinisk patologi
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- Johansson, Ann-Charlotte, 1976- (författare)
- Linköpings universitet,Centrum för social och affektiv neurovetenskap,Medicinska fakulteten
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- Edqvist, Per-Henrik D (författare)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Uppsala University, Uppsala, Sweden
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- Ponten, Fredrik (författare)
- Uppsala universitet,Klinisk och experimentell patologi,Uppsala University, Uppsala, Sweden
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- Roberg, Karin, 1957- (författare)
- Linköpings universitet,Avdelningen för cellbiologi,Medicinska fakulteten,Region Östergötland, Öron- näsa- och halskliniken US
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(creator_code:org_t)
- 2017-01-28
- 2017
- Engelska.
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Ingår i: Journal of Oral Pathology & Medicine. - : Wiley. - 0904-2512 .- 1600-0714. ; 46:9, s. 717-724
- Relaterad länk:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Ämnesord
Stäng
- BACKGROUND: The aims of this study were to validate in vitro drug sensitivity testing of head and neck squamous cell carcinoma (HNSCC) cell lines in an in vivo xenograft model and to identify treatment-induced changes in the epidermal growth factor receptor (EGFR) signaling pathway that could be used as markers for cetuximab treatment response.MATERIALS AND METHODS: The in vitro and in vivo cetuximab sensitivity of two HNSCC cell lines, UT-SCC-14 and UT-SCC-45, was assessed using a crystal violet assay and xenografts in nude mice, respectively. The expression of EGFR, phosphorylated EGFR (pEGFR), phosphorylated Src (pSrc), and Ki-67 was investigated by immunohistochemistry. To verify these results, the in vitro expression of EGFR and pEGFR was analyzed with ELISA in a panel of 10 HNSCC cell lines.RESULTS: A close correlation was found between in vitro and in vivo cetuximab sensitivity data in the two investigated HNSCC cell lines. In treatment sensitive UT-SCC-14 xenografts, there was a decrease in EGFR, pEGFR, and pSrc upon cetuximab treatment. Interestingly, in insensitive UT-SCC-45 xenografts, an increased expression of these three proteins was found. The change in EGFR and pEGFR expression in vivo was confirmed in cetuximab-sensitive and cetuximab-insensitive HNSCC cell lines using ELISA.CONCLUSION: High sensitivity to cetuximab was strongly associated with a treatment-induced reduction in pEGFR both in vivo and in vitro in a panel of HNSCC cell lines, suggesting that EGFR and pEGFR dynamics could be used as a predictive biomarker for cetuximab treatment response.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine (hsv//eng)
Nyckelord
- and xenografts
- cetuximab
- epidermal growth factor receptor
- head and neck squamous cell carcinoma
- phosphorylated epidermal growth factor receptor
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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