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Pro-inflammatory al...
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Fotaki, GrammatikiUppsala universitet,Science for Life Laboratory, SciLifeLab,Klinisk immunologi
(author)
Pro-inflammatory allogeneic DCs promote activation of bystander immune cells and thereby license antigen-specific T-cell responses
- Article/chapterEnglish2018
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LIBRIS-ID:oai:DiVA.org:uu-346363
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-346363URI
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https://doi.org/10.1080/2162402X.2017.1395126DOI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Accumulating evidence support an important role for endogenous bystander dendritic cells (DCs) in the efficiency of autologous patient-derived DC-vaccines, as bystander DCs take up material from vaccine-DCs, migrate to draining lymph node and initiate antitumor T-cell responses. We examined the possibility of using allogeneic DCs as vaccine-DCs to activate bystander immune cells and promote antigen-specific T-cell responses. We demonstrate that human DCs matured with polyI:C, R848 and IFN-γ (denoted COMBIG) in combination with an infection-enhanced adenovirus vector (denoted Ad5M) exhibit a pro-inflammatory state. COMBIG/Ad5M-matured allogeneic DCs (alloDCs) efficiently activated T-cells and NK-cells in allogeneic co-culture experiments. The secretion of immunostimulatory factors during the co-culture promoted the maturation of bystander-DCs, which efficiently cross-presented a model-antigen to activate antigen-specific CD8+ T-cells in vitro. We propose that alloDCs, in combination with Ad5M as loading vehicle, may be a cost-effective and logistically simplified DC vaccination strategy to induce anti-tumor immune responses in cancer patients.
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Jin, Chuan,1986-Uppsala universitet,Klinisk immunologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)chuji162
(author)
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Ramachandran, Mohanraj,1988-Uppsala universitet,Klinisk immunologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)mohra272
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Kerzeli, Iliana KyriakiUppsala universitet,Klinisk immunologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)iliky579
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Karlsson-Parra, AlexUppsala universitet,Klinisk immunologi,Science for Life Laboratory, SciLifeLab,Immunicum AB, Uppsala(Swepub:uu)aka12694
(author)
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Yu, Di,1985-Uppsala universitet,Klinisk immunologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)diayu422
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Essand, MagnusUppsala universitet,Klinisk immunologi,Science for Life Laboratory, SciLifeLab(Swepub:uu)magnessa
(author)
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Uppsala universitetScience for Life Laboratory, SciLifeLab
(creator_code:org_t)
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In:Oncoimmunology7:32162-40112162-402X
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