Sökning: onr:"swepub:oai:DiVA.org:uu-356449" > Development and wor...
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000 | 11224naa a2200985 4500 | |
001 | oai:DiVA.org:uu-356449 | |
003 | SwePub | |
008 | 180801s2018 | |||||||||||000 ||eng| | |
024 | 7 | a https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-3564492 URI |
024 | 7 | a https://doi.org/10.1038/s41598-018-24472-22 DOI |
040 | a (SwePub)uu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Fisher, Matthew C.u Imperial Coll London, Sch Publ Hlth, Fac Med, Dept Infect Dis Epidemiol, St Marys Campus, London W2 1PG, England4 aut |
245 | 1 0 | a Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi |
264 | c 2018-05-17 | |
264 | 1 | b NATURE PUBLISHING GROUP,c 2018 |
338 | a electronic2 rdacarrier | |
520 | a Parasitic chytrid fungi have emerged as a significant threat to amphibian species worldwide, necessitating the development of techniques to isolate these pathogens into culture for research purposes. However, early methods of isolating chytrids from their hosts relied on killing amphibians. We modified a pre-existing protocol for isolating chytrids from infected animals to use toe clips and biopsies from toe webbing rather than euthanizing hosts, and distributed the protocol to researchers as part of the BiodivERsA project RACE; here called the RML protocol. In tandem, we developed a lethal procedure for isolating chytrids from tadpole mouthparts. Reviewing a database of use a decade after their inception, we find that these methods have been applied across 5 continents, 23 countries and in 62 amphibian species. Isolation of chytrids by the non-lethal RML protocol occured in 18% of attempts with 207 fungal isolates and three species of chytrid being recovered. Isolation of chytrids from tadpoles occured in 43% of attempts with 334 fungal isolates of one species (Batrachochytrium dendrobatidis) being recovered. Together, these methods have resulted in a significant reduction and refinement of our use of threatened amphibian species and have improved our ability to work with this group of emerging pathogens. | |
650 | 7 | a NATURVETENSKAPx Biologix Mikrobiologi0 (SwePub)106062 hsv//swe |
650 | 7 | a NATURAL SCIENCESx Biological Sciencesx Microbiology0 (SwePub)106062 hsv//eng |
700 | 1 | a Ghosh, Priau Imperial Coll London, Sch Publ Hlth, Fac Med, Dept Infect Dis Epidemiol, St Marys Campus, London W2 1PG, England;North West Univ, Unit Environm Sci & Management, Private Bag x6001, ZA-2520 Potchefstroom, South Africa4 aut |
700 | 1 | a Shelton, Jennifer M. G.u Imperial Coll London, Sch Publ Hlth, Fac Med, Dept Infect Dis Epidemiol, St Marys Campus, London W2 1PG, England4 aut |
700 | 1 | a Bates, Kieranu Imperial Coll London, Sch Publ Hlth, Fac Med, Dept Infect Dis Epidemiol, St Marys Campus, London W2 1PG, England4 aut |
700 | 1 | a Brookes, Lolau Inst Zool, Regents Pk, London NW1 4RY, England4 aut |
700 | 1 | a Wierzbicki, Claudiau Imperial Coll London, Sch Publ Hlth, Fac Med, Dept Infect Dis Epidemiol, St Marys Campus, London W2 1PG, England4 aut |
700 | 1 | a Rosa, Goncalo M.u Inst Zool, Regents Pk, London NW1 4RY, England;Univ Lisbon, Fac Ciencias, Ctr Ecol Evolut & Environm Changes CE3C, Lisbon, Portugal4 aut |
700 | 1 | a Farrer, Rhys A.u Imperial Coll London, Sch Publ Hlth, Fac Med, Dept Infect Dis Epidemiol, St Marys Campus, London W2 1PG, England4 aut |
700 | 1 | a Aanensen, David M.u Imperial Coll London, Sch Publ Hlth, Fac Med, Dept Infect Dis Epidemiol, St Marys Campus, London W2 1PG, England;Ctr Genom Pathogen Surveillance, Wellcome Genome Campus, Hinxton, Cambs, England4 aut |
700 | 1 | a Alvarado-Rybak, Mariou Univ Andres Bello, Fac Ecol & Recursos Nat, Ctr Invest Sustentabilidad, Republ 440, Santiago, Chile4 aut |
700 | 1 | a Bataille, Arnaudu Seoul Natl Univ, Sch Biol Sci, Lab Behav & Populat Ecol, Seoul 08826, South Korea;CIRAD, UMR ASTRE, F-34398 Montpellier, France;Univ Montpellier, ASTRE, CIRAD, INRA, Montpellier, France4 aut |
700 | 1 | a Berger, Leeu James Cook Univ, Coll Publ Hlth Med & Vet Sci, Hlth Res Grp 1, Townsville, Qld 4811, Australia4 aut |
700 | 1 | a Böll, Susanneu Agcy Populat Ecol & Nat Conservancy, Gerbrunn, Germany4 aut |
700 | 1 | a Bosch, Jaimeu CSIC, Museo Nacl Ciencias Nat, C Jose Gutierrez Abascal 2, E-28006 Madrid, Spain4 aut |
700 | 1 | a Clare, Frances C.u Imperial Coll London, Sch Publ Hlth, Fac Med, Dept Infect Dis Epidemiol, St Marys Campus, London W2 1PG, England4 aut |
700 | 1 | a Courtois, Elodie A.u Univ Guyane, CNRS, IFREMER, LEEISA, Cayenne 97300, French Guiana4 aut |
700 | 1 | a Crottini, Angelicau Univ Porto, InBIO, CIBIO Ctr Invest Biodiversidade & Recursos Genet, P-4485661 Vairao, Portugal4 aut |
700 | 1 | a Cunningham, Andrew A.u Inst Zool, Regents Pk, London NW1 4RY, England4 aut |
700 | 1 | a Doherty-Bone, Thomas M.u Royal Zool Soc Scotland, Conservat Programmes, Edinburgh, Midlothian, Scotland4 aut |
700 | 1 | a Gebresenbet, Fikirteu Oklahoma State Univ, Dept Integrat Biol, Life Sci West 113, Stillwater, OK 74078 USA4 aut |
700 | 1 | a Gower, David J.u Nat Hist Museum, Life Sci, London SW7 5BD, England4 aut |
700 | 1 | a Höglund, Jacobu Uppsala universitet,Zooekologi4 aut0 (Swepub:uu)jacohogl |
700 | 1 | a James, Timothy Y.u Univ Michigan, Dept Ecol & Evolutionary Biol, Ann Arbor, MI 48109 USA4 aut |
700 | 1 | a Jenkinson, Thomas S.u Univ Michigan, Dept Ecol & Evolutionary Biol, Ann Arbor, MI 48109 USA4 aut |
700 | 1 | a Kosch, Tiffany A.u Seoul Natl Univ, Sch Biol Sci, Lab Behav & Populat Ecol, Seoul 08826, South Korea;James Cook Univ, Coll Publ Hlth Med & Vet Sci, Hlth Res Grp 1, Townsville, Qld 4811, Australia4 aut |
700 | 1 | a Lambertini, Carolinau Univ Estadual Campinas, Inst Biol, Dept Biol Anim, Lab Hist Nat Anfibios Brasileiros, BR-13083862 Campinas, SP, Brazil4 aut |
700 | 1 | a Laurila, Anssiu Uppsala universitet,Zooekologi4 aut0 (Swepub:uu)ansslaur |
700 | 1 | a Lin, Chun-Fuu Endem Species Res Inst, Zool Div, 1 Ming Shen East Rd, Nantou 552, Taiwan4 aut |
700 | 1 | a Loyau, Adelineu UFZ Helmholtz Ctr Environm Res, Dept Conservat Biol, Permoserstr 15, D-04318 Leipzig, Germany;Univ Toulouse, CNRS, ECOLAB, INPT,UPS, Toulouse, France4 aut |
700 | 1 | a Martel, Anu Univ Ghent, Dept Pathol Bacteriol & Avian Dis, Fac Vet Med, Salisburylaan 133, B-9820 Merelbeke, Belgium4 aut |
700 | 1 | a Meurling, Sarau Uppsala universitet,Zooekologi4 aut0 (Swepub:uu)sarme202 |
700 | 1 | a Miaud, Claudeu Univ Paul Valery Montpellier, Univ Montpellier, PSL Res Univ, CEFE,UMR 5175,CNRS,EPHE,Biogeog & Ecol Vertebres, Montpellier, France4 aut |
700 | 1 | a Minting, Peteu Amphibian & Reptile Conservat ARC Trust, 655A Christchurch Rd, Bournemouth BH1 4AP, Dorset, England4 aut |
700 | 1 | a Ndriantsoa, Sergeu Durrell Wildlife Conservat Trust, Madagascar Programme, Antananarivo, Madagascar4 aut |
700 | 1 | a O'Hanlon, Simon J.u Imperial Coll London, Sch Publ Hlth, Fac Med, Dept Infect Dis Epidemiol, St Marys Campus, London W2 1PG, England;Inst Zool, Regents Pk, London NW1 4RY, England4 aut |
700 | 1 | a Pasmans, Franku Univ Ghent, Dept Pathol Bacteriol & Avian Dis, Fac Vet Med, Salisburylaan 133, B-9820 Merelbeke, Belgium4 aut |
700 | 1 | a Rakotonanahary, Tsantau Durrell Wildlife Conservat Trust, Madagascar Programme, Antananarivo, Madagascar4 aut |
700 | 1 | a Rabemananjara, Falitiana C. E.u Durrell Wildlife Conservat Trust, Madagascar Programme, Antananarivo, Madagascar;IUCN SSC Amphibian Specialist Grp Madagascar, Antananarivo 101, Madagascar4 aut |
700 | 1 | a Ribeiro, Luisa P.u Univ Estadual Campinas, Inst Biol, Dept Biol Anim, Lab Hist Nat Anfibios Brasileiros, BR-13083862 Campinas, SP, Brazil4 aut |
700 | 1 | a Schmeller, Dirk S.u UFZ Helmholtz Ctr Environm Res, Dept Conservat Biol, Permoserstr 15, D-04318 Leipzig, Germany;Univ Toulouse, CNRS, ECOLAB, INPT,UPS, Toulouse, France4 aut |
700 | 1 | a Schmidt, Benedikt R.u Univ Zurich, Dept Evolutionary Biol & Environm Studies, Winterthurerstr 190, CH-8057 Zurich, Switzerland;Univ Neuchatel, Info Fauna Karch, Bellevaux 51,UniMail Batiment 6, CH-2000 Neuchatel, Switzerland4 aut |
700 | 1 | a Skerratt, Leeu James Cook Univ, Coll Publ Hlth Med & Vet Sci, Hlth Res Grp 1, Townsville, Qld 4811, Australia4 aut |
700 | 1 | a Smith, Freyau APHA, Natl Wildlife Management Ctr, Woodchester Pk GL10 3UJ, Glos, England4 aut |
700 | 1 | a Soto-Azat, Claudiou Univ Andres Bello, Fac Ecol & Recursos Nat, Ctr Invest Sustentabilidad, Republ 440, Santiago, Chile4 aut |
700 | 1 | a Tessa, Giuliau Nonprofit Assoc Zirichiltaggi Sardinia Wildlife C, Str Vicinale Filigheddu 62-C, I-07100 Sassari, Italy4 aut |
700 | 1 | a Toledo, Luis Felipeu Univ Estadual Campinas, Inst Biol, Dept Biol Anim, Lab Hist Nat Anfibios Brasileiros, BR-13083862 Campinas, SP, Brazil4 aut |
700 | 1 | a Valenzuela-Sanchez, Andresu Univ Andres Bello, Fac Ecol & Recursos Nat, Ctr Invest Sustentabilidad, Republ 440, Santiago, Chile;ONG Ranita Darwin, Nataniel Cox 152, Santiago, Chile4 aut |
700 | 1 | a Verster, Ruhanu North West Univ, Unit Environm Sci & Management, Private Bag x6001, ZA-2520 Potchefstroom, South Africa4 aut |
700 | 1 | a Vörös, Juditu Hungarian Nat Hist Museum, Dept Zool, Collect Amphibians & Reptiles, Baross U 13, H-1088 Budapest, Hungary4 aut |
700 | 1 | a Waldman, Bruceu Seoul Natl Univ, Sch Biol Sci, Lab Behav & Populat Ecol, Seoul 08826, South Korea4 aut |
700 | 1 | a Webb, Rebecca J.u James Cook Univ, Coll Publ Hlth Med & Vet Sci, Hlth Res Grp 1, Townsville, Qld 4811, Australia4 aut |
700 | 1 | a Weldon, Cheu North West Univ, Unit Environm Sci & Management, Private Bag x6001, ZA-2520 Potchefstroom, South Africa4 aut |
700 | 1 | a Wombwell, Emmau Inst Zool, Regents Pk, London NW1 4RY, England4 aut |
700 | 1 | a Zamudio, Kelly R.u Cornell Univ, Dept Ecol & Evolutionary Biol, Ithaca, NY 14853 USA4 aut |
700 | 1 | a Longcore, Joyce E.u Univ Maine, Sch Biol & Ecol, Orono, ME 04469 USA4 aut |
700 | 1 | a Garner, Trenton W. J.u Inst Zool, Regents Pk, London NW1 4RY, England;Nonprofit Assoc Zirichiltaggi Sardinia Wildlife C, Str Vicinale Filigheddu 62-C, I-07100 Sassari, Italy;North West Univ, Unit Environm Sci & Management, Private Bag x6001, ZA-2520 Potchefstroom, South Africa4 aut |
710 | 2 | a Imperial Coll London, Sch Publ Hlth, Fac Med, Dept Infect Dis Epidemiol, St Marys Campus, London W2 1PG, Englandb Imperial Coll London, Sch Publ Hlth, Fac Med, Dept Infect Dis Epidemiol, St Marys Campus, London W2 1PG, England;North West Univ, Unit Environm Sci & Management, Private Bag x6001, ZA-2520 Potchefstroom, South Africa4 org |
773 | 0 | t Scientific Reportsd : NATURE PUBLISHING GROUPg 8q 8x 2045-2322 |
856 | 4 | u https://doi.org/10.1038/s41598-018-24472-2y Fulltext |
856 | 4 | u https://uu.diva-portal.org/smash/get/diva2:1236282/FULLTEXT01.pdfx primaryx Raw objecty fulltext:print |
856 | 4 | u https://www.nature.com/articles/s41598-018-24472-2.pdf |
856 | 4 8 | u https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-356449 |
856 | 4 8 | u https://doi.org/10.1038/s41598-018-24472-2 |
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