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Luminescent CeO2:Eu...
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Henning, Dorian F.Karolinska Inst, Dept Microbiol Tumor & Cell Biol, SE-17177 Stockholm, Sweden;Imperial Coll London, Dept Comp, London, England
(författare)
Luminescent CeO2:Eu3+ nanocrystals for robust in situ H2O2 real-time detection in bacterial cell cultures
- Artikel/kapitelEngelska2019
Förlag, utgivningsår, omfång ...
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ELSEVIER ADVANCED TECHNOLOGY,2019
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printrdacarrier
Nummerbeteckningar
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LIBRIS-ID:oai:DiVA.org:uu-382822
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-382822URI
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https://doi.org/10.1016/j.bios.2019.03.012DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:140710377URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
Anmärkningar
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Hydrogen peroxide (H2O2) quantification in biomedicine is valuable as inflammation biomarker but also in assays employing enzymes that generate or consume H2O2 linked to a specific biomarker. Optical H2O2 detection is typically performed through peroxidase-coupled reactions utilizing organic dyes that suffer, however, from poor stability/reproducibility and also cannot be employed in situ in dynamic complex cell cultures to monitor H2O2 levels in real-time. Here, we utilize enzyme-mimetic CeO2 nanocrystals that are sensitive to H2O2 and study the effect of H2O2 presence on their electronic and luminescent properties. We produce and dope with Eu3+ these particles in a single-step by flame synthesis and directly deposit them on Si and glass substrates to fabricate nanoparticle layers to monitor in real-time and in situ the H2O2 concentrations generated by Streptococcus pneumoniae clinical isolates. Furthermore, the small CeO2:Eu3+ nanocrystals are combined in a single-step with larger, non-responsive Y2O3:Tb3+ nanoparticles during their double-nozzle flame synthesis to engineer hybrid luminescent nanoaggregates as ratiometric robust biosensors. We demonstrate the functionality of these biosensors by monitoring their response in the presence of a broad range of H2O2 concentrations in vitro from S. pneumoniae, highlighting their potential for facile real-time H2O2 detection in vitro in cell cultures.
Ämnesord och genrebeteckningar
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Merkl, PadrykKarolinska Institutet
(författare)
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Yun, ChanghunKarolinska Inst, Dept Microbiol Tumor & Cell Biol, SE-17177 Stockholm, Sweden
(författare)
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Iovino, FedericoKarolinska Institutet
(författare)
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Xie, Ling,1982-Uppsala universitet,Tillämpad materialvetenskap(Swepub:uu)xieli203
(författare)
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Mouzourakis, EleftheriosUniv Ioannina, Dept Phys, GR-45110 Ioannina, Greece
(författare)
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Moularas, ConstantinosUniv Ioannina, Dept Phys, GR-45110 Ioannina, Greece
(författare)
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Deligiannakis, YiannisUniv Ioannina, Dept Phys, GR-45110 Ioannina, Greece
(författare)
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Henriques-Normark, BirgittaKarolinska Institutet
(författare)
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Leifer, Klaus,1965-Uppsala universitet,Tillämpad materialvetenskap(Swepub:uu)kllei839
(författare)
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Sotiriou, Georgios A.Karolinska Institutet
(författare)
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Karolinska InstitutetKarolinska Inst, Dept Microbiol Tumor & Cell Biol, SE-17177 Stockholm, Sweden;Imperial Coll London, Dept Comp, London, England
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Biosensors & bioelectronics: ELSEVIER ADVANCED TECHNOLOGY132, s. 286-2930956-56631873-4235
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Henning, Dorian ...
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Merkl, Padryk
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Yun, Changhun
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Iovino, Federico
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Xie, Ling, 1982-
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Mouzourakis, Ele ...
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Moularas, Consta ...
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Deligiannakis, Y ...
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Henriques-Normar ...
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Leifer, Klaus, 1 ...
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Sotiriou, Georgi ...
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Uppsala universitet
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