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- Bergman, DanielSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences,Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden (author)
Investigation of interference from canine anti-mouse antibodies in hormone immunoassays
- Article/chapterEnglish2019
Publisher, publication year, extent ...
- 2019-07-18
- Wiley,2019
- electronicrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:uu-389639
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-389639URI
- https://doi.org/10.1111/vcp.12764DOI
- https://res.slu.se/id/publ/100741URI
Supplementary language notes
- Language:English
- Summary in:English
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- SwePubSwePub
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Notes
- BACKGROUND: Canine anti-mouse antibodies are a potential source of immunoassay interference, but erroneous immunoassay results are not always easily identifiable. Anti-Müllerian hormone (AMH) is a marker for the presence of gonads in dogs, but elevated AMH concentrations in neutered dogs could also be caused by antibody interference. For other assays, a discrepant result obtained after antibody precipitation might indicate antibody interference.OBJECTIVES: We aimed to evaluate if canine anti-mouse antibodies are a source of erroneous results in the AMH assay and if antibody precipitation with polyethylene glycol (PEG) is a useful tool for detecting antibody interference in a variety of immunoassays used in the veterinary clinical laboratory.METHODS: Twenty-nine positive and 25 negative samples for anti-mouse antibodies were analyzed for AMH, canine total thyroxine (TT4), canine thyroid-stimulating hormone (TSH) and progesterone before and after treatment with PEG. Results that differed by more than four SDs from the intra-assay coefficients of variation were considered discrepant. Elevated AMH concentrations in neutered dogs with anti-mouse antibodies and no visible gonads present were considered evidence of interference.RESULTS: Evidence of antibody interference was found in two samples analyzed for AMH. The presence of anti-mouse antibodies did not lead to a higher proportion of discrepant results after PEG treatment for any of the immunoassays. The overall incidence of discrepant results for healthy controls was very high (73%).CONCLUSIONS: Canine anti-mouse antibodies are a source of erroneous AMH results. Antibody precipitation with PEG is not a useful tool for detecting interference caused by such antibodies.
Subject headings and genre
- LANTBRUKSVETENSKAPER Veterinärmedicin Klinisk vetenskap hsv//swe
- AGRICULTURAL SCIENCES Veterinary Science Clinical Science hsv//eng
- MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Endokrinologi och diabetes hsv//swe
- MEDICAL AND HEALTH SCIENCES Clinical Medicine Endocrinology and Diabetes hsv//eng
- MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Klinisk laboratoriemedicin hsv//swe
- MEDICAL AND HEALTH SCIENCES Clinical Medicine Clinical Laboratory Medicine hsv//eng
- PEG
- anti-Müllerian hormone
- antibody
- canine
- polyethylene glycol
Added entries (persons, corporate bodies, meetings, titles ...)
- Larsson, AndersUppsala universitet,Klinisk kemi(Swepub:uu)andlarss (author)
- Hamlin, HeleneSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences,Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden(Swepub:slu)50428 (author)
- Ström Holst, BodilSwedish University of Agricultural Sciences,Sveriges lantbruksuniversitet,Institutionen för kliniska vetenskaper (KV),Department of Clinical Sciences,Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden(Swepub:slu)47321 (author)
- Sveriges lantbruksuniversitetInstitutionen för kliniska vetenskaper (KV) (creator_code:org_t)
- Sveriges lantbruksuniversitet
Related titles
- In:Veterinary clinical pathology: Wiley48:S1, s. 59-690275-63821939-165X
Internet link
- https://doi.org/10.1111/vcp.12764Fulltext
- https://uu.diva-portal.org/smash/get/diva2:1338041/FULLTEXT01.pdffulltext:print
- https://onlinelibrary.wiley.com/doi/pdfdirect/10.1111/vcp.12764
- https://pub.epsilon.slu.se/id/eprint/16384/contentsFULLTEXT
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-389639
- https://doi.org/10.1111/vcp.12764
- https://res.slu.se/id/publ/100741
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