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Oligosaccharides ma...
Oligosaccharides mapping of nitrous acid degraded heparin through UHPLC-HILIC/WAX-MS
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- Zhang, Tianji (author)
- Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing, Peoples R China;Natl Inst Metrol, Div Chem & Analyt Sci, Beijing, Peoples R China
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- Xie, Shaoshuai (author)
- Shandong Univ, Natl Glycoengn Res Ctr, Jinan, Shandong, Peoples R China
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- Wang, Zhangjie (author)
- Shandong Univ, Natl Glycoengn Res Ctr, Jinan, Shandong, Peoples R China
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- Zhang, Rui (author)
- Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing, Peoples R China
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- Sun, Qianyun (author)
- Shandong Inst Metrol, Div Chem, Jinan, Shandong, Peoples R China
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- Liu, Xin (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Chi, Lianli (author)
- Shandong Univ, Natl Glycoengn Res Ctr, Jinan, Shandong, Peoples R China
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- Li, Jin-Ping (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Beijing Univ Chem Technol, Int Res Ctr Soft Matter, Beijing, Peoples R China
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- Li, Hongmei (author)
- Natl Inst Metrol, Div Chem & Analyt Sci, Beijing, Peoples R China
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- Tan, Tianwei (author)
- Beijing Univ Chem Technol, Coll Life Sci & Technol, Beijing, Peoples R China
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(creator_code:org_t)
- Elsevier, 2020
- 2020
- English.
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In: Carbohydrate Polymers. - : Elsevier. - 0144-8617 .- 1879-1344. ; 231
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https://doi.org/10.1...
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Abstract
Subject headings
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- Building blocks characterization is a significant approach for understanding the molecular structure of heparin and its derivatives. Nitrous acid (HONO) depolymerization of heparin generates oligosaccharides that maintain the epimerization conformation on C5 of the uronic acids, reflecting the authentic structure of the parental chain. HONO treatment at pH 1.5 selectively cleaves the bond between N-sulfated glucosamine and hexuronic acid, resulting mainly disaccharides, as well as tetra-, tri-, and mono-saccharides. The tetrasaccharides are derived from the structure of N-acetylated domains while tri-, and mono-saccharides are derived from the reducing or the non-reducing end of the heparin chain. The resulted oligosaccharides were separated and analyzed using a UHPLC-HILIC/WAX-MS method. We succeeded in the identification of 19 tetrasaccharides, 19 trisaccharides and 4 monosaccharides species, majority of which is structurally characterized. By comparing the theoretical possibilities and actual occurrence of the well-characterized tetrasaccharides, we demonstrated that the biosynthesis of heparin is a systematic process.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Keyword
- Heparin
- Tetrasaccharide
- Trisaccharide
- Monosaccharide
- UHPLC-HILIC/WAX-MS/MS
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Zhang, Tianji
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Xie, Shaoshuai
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Wang, Zhangjie
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Zhang, Rui
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Sun, Qianyun
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Liu, Xin
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show more...
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Chi, Lianli
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Li, Jin-Ping
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Li, Hongmei
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Tan, Tianwei
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Analytical Chemi ...
- Articles in the publication
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Carbohydrate Pol ...
- By the university
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Uppsala University