Comparable cancer-relevant mutation profiles in synchronous ductal carcinoma in situ and invasive breast cancer

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Bergholtz, HelgaOslo Univ Hosp, Inst Canc Res, Dept Canc Genet, Oslo, Norway.;Univ Oslo, Inst Clin Med, Fac Med, Oslo, Norway. (författare)

Comparable cancer-relevant mutation profiles in synchronous ductal carcinoma in situ and invasive breast cancer

Artikel/kapitelEngelska2020

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2020-05-28
WILEY,2020
electronicrdacarrier

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LIBRIS-ID:oai:DiVA.org:uu-426309
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-426309URI
https://doi.org/10.1002/cnr2.1248DOI

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Språk:engelska
Sammanfattning på:engelska

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Background: Ductal carcinoma in situ (DCIS) comprises a diverse group of preinvasive lesions in the breast and poses a considerable clinical challenge due to lack of markers of progression. Genomic alterations are to a large extent similar in DCIS and invasive carcinomas, although differences in copy number aberrations, gene expression patterns, and mutations exist. In mixed tumors with synchronous invasive breast cancer (IBC) and DCIS, it is still unclear to what extent invasive tumor cells are directly derived from the DCIS cells.Aim: Our aim was to compare cancer-relevant mutation profiles of different cellular compartments in mixed DCIS/IBC and pure DCIS tumors.Methods and results: We performed targeted sequencing of 50 oncogenes in microdissected tissue from three different epithelial cell compartments (in situ, invasive, and normal adjacent epithelium) from 26 mixed breast carcinomas. In total, 44 tissue samples (19 invasive, 16 in situ, 9 normal) were subjected to sequencing using the Ion Torrent platform and the AmpliSeq Cancer Hotspot Panel v2. For comparison, 10 additional, pure DCIS lesions were sequenced. Across all mixed samples, we detected 23 variants previously described in cancer. The most commonly affected genes were TP53, PIK3CA, and ERBB2. The PIK3CA:p.H1047R variant was found in nine samples from six patients. Most variants detected in invasive compartments were also found in the corresponding in situ cell compartment indicating a clonal relationship between the tumor stages. A lower frequency of variants were observed in pure DCIS lesions.Conclusion: Similar mutation profiles between in situ and invasive cell compartments indicate a similar origin of the two tumor stages in mixed breast tumors. The lower number of potential driver variants found in pure DCIS compared with the in situ cell compartments of mixed tumors may imply that pure DCIS is captured earlier in the path of progression to invasive disease.

Ämnesord och genrebeteckningar

MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Cancer och onkologi hsv//swe
MEDICAL AND HEALTH SCIENCES Clinical Medicine Cancer and Oncology hsv//eng
breast tumor progression
DCIS
invasive breast cancer
mutations
targeted sequencing

Biuppslag (personer, institutioner, konferenser, titlar ...)

Kumar, SurendraOslo Univ Hosp, Inst Canc Res, Dept Canc Genet, Oslo, Norway. (författare)
Wärnberg, FredrikUppsala universitet,Endokrinkirurgi(Swepub:uu)fredwarn (författare)
Lüders, TorbenUniv Oslo, Inst Clin Med, Fac Med, Oslo, Norway.;Akershus Univ Hosp, Div Med, Dept Clin Mol Biol EpiGen, Lorenskog, Norway. (författare)
Kristensen, VesselaOslo Univ Hosp, Inst Canc Res, Dept Canc Genet, Oslo, Norway.;Univ Oslo, Inst Clin Med, Fac Med, Oslo, Norway.;Akershus Univ Hosp, Div Med, Dept Clin Mol Biol EpiGen, Lorenskog, Norway. (författare)
Sörlie, ThereseOslo Univ Hosp, Inst Canc Res, Dept Canc Genet, Oslo, Norway.;Univ Oslo, Inst Clin Med, Fac Med, Oslo, Norway.;Akershus Univ Hosp, Div Med, Dept Clin Mol Biol EpiGen, Lorenskog, Norway. (författare)
Oslo Univ Hosp, Inst Canc Res, Dept Canc Genet, Oslo, Norway.;Univ Oslo, Inst Clin Med, Fac Med, Oslo, Norway.Oslo Univ Hosp, Inst Canc Res, Dept Canc Genet, Oslo, Norway. (creator_code:org_t)

Sammanhörande titlar

Ingår i:Cancer Reports: WILEY3:32573-8348

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Av författaren/redakt...: Bergholtz, Helga; Kumar, Surendra; Wärnberg, Fredri ...; Lüders, Torben; Kristensen, Vess ...; Sörlie, Therese

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MEDICIN OCH HÄLSOVETENSKAP: MEDICIN OCH HÄLS ...; och Klinisk medicin; och Cancer och onkol ...

Artiklar i publikationen: Cancer Reports

Av lärosätet: Uppsala universitet

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