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Unrelated Fungal Ru...
Unrelated Fungal Rust Candidate Effectors Act on Overlapping Plant Functions
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- Goncalves dos Santos, Karen Cristine (författare)
- Univ Quebec Trois Rivieres, Dept Chem Biochem & Phys, Trois Rivieres, PQ G9A 5H9, Canada; Univ Quebec Trois Rivieres, Plant Biol Res Grp, Trois Rivieres, PQ G8Z 1V3, Canada
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- Pelletier, Gervais (författare)
- Laurentian Forestry Ctr, Canadian Forest Serv, Nat Resources Canada, Quebec City, PQ G1V 4C7, Canada
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- Séguin, Armand (författare)
- Laurentian Forestry Ctr, Canadian Forest Serv, Nat Resources Canada, Quebec City, PQ G1V 4C7, Canada
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- Guillemette, François (författare)
- Univ Quebec Trois Rivieres, Ctr Res Aquat Ecosyst Interact Rive, Trois Rivieres, PQ G8Z 1V3, Canada
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- Hawkes, Jeffrey (författare)
- Uppsala universitet,Analytisk kemi
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- Desgagné-Penix, Isabel (författare)
- Univ Quebec Trois Rivieres, Dept Chem Biochem & Phys, Trois Rivieres, PQ G9A 5H9, Canada; Univ Quebec Trois Rivieres, Plant Biol Res Grp, Trois Rivieres, PQ G8Z 1V3, Canada
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- Germain, Hugo (författare)
- Univ Quebec Trois Rivieres, Dept Chem Biochem & Phys, Trois Rivieres, PQ G9A 5H9, Canada; Univ Quebec Trois Rivieres, Plant Biol Res Grp, Trois Rivieres, PQ G8Z 1V3, Canada
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(creator_code:org_t)
- 2021-05-05
- 2021
- Engelska.
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Ingår i: Microorganisms. - : MDPI. - 2076-2607. ; 9:5
- Relaterad länk:
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https://doi.org/10.3...
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https://uu.diva-port... (primary) (Raw object)
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https://www.mdpi.com...
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https://urn.kb.se/re...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Rust fungi cause epidemics that threaten the production of important plant species, such as wheat and soy. Melampsora larici-populina (Mlp) causes the poplar rust and encodes at least 1184 candidate effectors (CEs) whose functions are poorly known. In this study, we sequenced the transcriptome and used mass spectrometry to analyze the metabolome of Arabidopsis plants constitutively expressing 14 Mlp CEs and of a control line to discover alterations leading to plant susceptibility. We found 2299 deregulated genes across the experiment. Genes involved in pattern-triggered immunity, such as FRK1, PR1, RBOHD, and WRKY33, as well as AUX/IAA genes were down-regulated. We further observed that 680 metabolites were deregulated in at least one CE-expressing transgenic line, with “highly unsaturated and phenolic compounds” and “peptides” enriched among down- and up-regulated metabolites. Interestingly, transgenic lines expressing unrelated CEs had correlated patterns of gene and metabolite deregulation, while expression of CEs belonging to the same family deregulated different genes and metabolites. Thus, our results uncouple effector sequence similarity and function. This supports that effector functional investigation in the context of their virulence activity and effect on plant susceptibility requires the investigation of the individual effector and precludes generalization based on sequence similarity.
Ämnesord
- LANTBRUKSVETENSKAPER -- Bioteknologi med applikationer på växter och djur -- Växtbioteknologi (hsv//swe)
- AGRICULTURAL SCIENCES -- Agricultural Biotechnology -- Plant Biotechnology (hsv//eng)
Nyckelord
- transcriptome
- metabolome
- plant-microbe interactions
- rust fungi
- effector biology
- Melampsora larici-populina
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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