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Spatial visualizati...
Spatial visualization of comprehensive brain neurotransmitter systems and neuroactive substances by selective in situ chemical derivatization mass spectrometry imaging
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- Shariatgorji, Reza (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Science for Life Laboratory, SciLifeLab
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- Nilsson, Anna (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Science for Life Laboratory, SciLifeLab
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- Fridjonsdottir, Elva (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap
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- Strittmatter, Nicole (author)
- AstraZeneca, BioPharmaceut R&D, Clin Pharmacol & Safety Sci, Imaging & Data Analyt, Cambridge, England.
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- Dannhorn, Andreas (author)
- AstraZeneca, BioPharmaceut R&D, Clin Pharmacol & Safety Sci, Imaging & Data Analyt, Cambridge, England.
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- Svenningsson, Per (author)
- Karolinska Institutet
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- Goodwin, Richard J. A. (author)
- AstraZeneca, BioPharmaceut R&D, Clin Pharmacol & Safety Sci, Imaging & Data Analyt, Cambridge, England.
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- Odell, Luke R. (author)
- Uppsala universitet,Preparativ läkemedelskemi
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- Andrén, Per E., Professor, 1957- (author)
- Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Science for Life Laboratory, SciLifeLab
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(creator_code:org_t)
- 2021-06-02
- 2021
- English.
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In: Nature Protocols. - : Springer Nature. - 1754-2189 .- 1750-2799. ; 16:7, s. 3298-3321
- Related links:
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Abstract
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- Molecule-specific techniques such as MALDI and desorption electrospray ionization mass spectrometry imaging enable direct and simultaneous mapping of biomolecules in tissue sections in a single experiment. However, neurotransmitter imaging in the complex environment of biological samples remains challenging. Our covalent charge-tagging approach using on-tissue chemical derivatization of primary and secondary amines and phenolic hydroxyls enables comprehensive mapping of neurotransmitter networks. Here, we present robust and easy-to-use chemical derivatization protocols that facilitate quantitative and simultaneous molecular imaging of complete neurotransmitter systems and drugs in diverse biological tissue sections with high lateral resolution. This is currently not possible with any other imaging technique. The protocol, using fluoromethylpyridinium and pyrylium reagents, describes all steps from tissue preparation (-1 h), chemical derivatization (1-2 h), data collection (timing depends on the number of samples and lateral resolution) and data analysis and interpretation. The specificity of the chemical reaction can also help users identify unknown chemical identities. Our protocol can reveal the cellular locations in which signaling molecules act and thus shed light on the complex responses that occur after the administration of drugs or during the course of a disease.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Shariatgorji, Re ...
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Nilsson, Anna
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Fridjonsdottir, ...
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Strittmatter, Ni ...
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Dannhorn, Andrea ...
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Svenningsson, Pe ...
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Goodwin, Richard ...
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Odell, Luke R.
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Andrén, Per E., ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Analytical Chemi ...
- Articles in the publication
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Nature Protocols
- By the university
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Uppsala University
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Karolinska Institutet