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Use of metagenomic ...
Use of metagenomic microbial source tracking to investigate the source of a foodborne outbreak of cryptosporidiosis
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- Ahlinder, J. (författare)
- FOI, Swedish Def Res Agcy, CBRN Def & Secur, Umeå, Sweden.
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- Svedberg, A-L (författare)
- Reg Norrbotten, Dept Infect Control, Luleå, Sweden.
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- Nystedt, A. (författare)
- Reg Norrbotten, Dept Infect Control, Luleå, Sweden.
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- Dryselius, R. (författare)
- Natl Food Agcy, Dept Biol, Uppsala, Sweden.
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- Jacobsson, K. (författare)
- Natl Food Agcy, Dept Biol, Uppsala, Sweden.
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- Hägglund, M. (författare)
- Clin Genom Facil, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden.
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- Brindefalk, B. (författare)
- FOI, Swedish Def Res Agcy, CBRN Def & Secur, Umeå, Sweden.
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- Forsman, M. (författare)
- FOI, Swedish Def Res Agcy, CBRN Def & Secur, Umeå, Sweden.
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- Ottoson, J. (författare)
- Natl Food Agcy, Dept Risk & Benefit Assessment, Uppsala, Sweden.
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- Troell, Karin (författare)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Natl Vet Inst, Dept Microbiol, Uppsala, Sweden.
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FOI, Swedish Def Res Agcy, CBRN Def & Secur, Umeå, Sweden Reg Norrbotten, Dept Infect Control, Luleå, Sweden. (creator_code:org_t)
- Elsevier, 2022
- 2022
- Engelska.
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Ingår i: FOOD AND WATERBORNE PARASITOLOGY. - : Elsevier. - 2405-6766. ; 26
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://doi.org/10.1...
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Cryptosporidium is a protozoan parasite of global public health importance that causes gastroenteritis in a variety of vertebrate hosts, with many human outbreaks reported yearly, often from ingestion of contaminated water or food. Despite the major public health implications, little is typically known about sources of contamination of disease outbreaks caused by Cryptosporidium. Here, we study a national foodborne outbreak resulted from infection with Cryptosporidium parvum via romaine lettuce, with the main goal to trace the source of the parasite. To do so, we combined traditional outbreak investigation methods with molecular detection and characterization methods (i.e. PCR based typing, amplicon and shotgun sequencing) of romaine lettuce samples collected at the same farm from which the contaminated food was produced. Using 18S rRNA typing, we detected C. parvum in two out of three lettuce samples, which was supported by detections in the metagenome analysis. Microbial source tracking analysis of the lettuce samples suggested sewage water as a likely source of the contamination, albeit with some uncertainty. In addition, the high degree of overlap in bacterial species content with a public human gut microbial database corroborated the source tracking results. The combination of traditional and molecular based methods applied here is a promising tool for future source tracking investigations of food- and waterborne outbreaks of Cryptosporidium spp. and can help to control and mitigate contamination risks.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
Nyckelord
- Vegetables
- Contaminated food
- Cryptosporidium parvum
- Foodborne outbreak investigation
- 18S rRNA
- 16S rRNA
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Ahlinder, J.
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Svedberg, A-L
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Nystedt, A.
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Dryselius, R.
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Jacobsson, K.
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Hägglund, M.
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visa fler...
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Brindefalk, B.
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Forsman, M.
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Ottoson, J.
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Troell, Karin
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visa färre...
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- Av lärosätet
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Uppsala universitet