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The glycine recepto...
The glycine receptor alpha 3 subunit mRNA expression shows sex-dependent differences in the adult mouse brain
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- Ceder, Mikaela M. (författare)
- Uppsala universitet,Genomik och neurobiologi,Malin Lagerström
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- Weman, Hannah M., 1993- (författare)
- Uppsala universitet,Genomik och neurobiologi,Malin Lagerström
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- Johansson, Ebba (författare)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi
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- Henriksson, Katharina, 1993- (författare)
- Uppsala universitet,Genomik och neurobiologi,Malin Lagerström
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- Magnusson, Kajsa A., 1997- (författare)
- Uppsala universitet,Genomik och neurobiologi,Malin Lagerström
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- Roman, Erika (författare)
- Swedish University of Agricultural Sciences,Uppsala universitet,Institutionen för farmaceutisk biovetenskap,Sveriges lantbruksuniversitet, institutionen för anatomi, fysiologi och biokemi,Institutionen för anatomi, fysiologi och biokemi (AFB),Department of Anatomy, Physiology and Biochemistry (AFB),Uppsala University
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- Lagerström, Malin C. (författare)
- Uppsala universitet,Genomik och neurobiologi,Malin Lagerström
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(creator_code:org_t)
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- Springer Nature, 2023
- 2023
- Engelska.
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Ingår i: BMC Neuroscience. - : Springer Nature. - 1471-2202. ; 24:1
- Relaterad länk:
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https://doi.org/10.1...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Background The glycinergic system plays an important inhibitory role in the mouse central nervous system, where glycine controls the excitability of spinal itch- and pain-mediating neurons. Impairments of the glycine receptors can cause motor and sensory deficits. Glycine exerts inhibition through interaction with ligand-gated ion channels composed of alpha and beta subunits. We have investigated the mRNA expression of the glycine receptor alpha 3 (Glra3) subunit in the nervous system as well as in several peripheral organs of female and male mice.Results Single-cell RNA sequencing (scRNA-seq) data analysis on the Zeisel et al. (2018) dataset indicated widespread but low expression of Glra3 in vesicular glutamate transporter 2 (Vglut2, Slc17a6) positive and vesicular inhibitory amino acid transporter (Viaat, Slc32a1)positive neurons of the mouse central nervous system. Highest occurrence of Glra3 expression was identified in the cortex, amygdala, and striatal regions, as well as in the hypothalamus, brainstem and spinal cord. Bulk quantitative real-time-PCR (qRT-PCR) analysis demonstrated Glra3 expression in cortex, amygdala, striatum, hypothalamus, thalamus, pituitary gland, hippocampus, cerebellum, brainstem, and spinal cord. Additionally, male mice expressed higher levels of Glra3 in all investigated brain areas compared with female mice. Lastly, RNAscope spatially validated Glra3 expression in the areas indicated by the single-cell and bulk analyses. Moreover, RNAscope analysis confirmed co-localization of Glra3 with Slc17a6 or Slc32a1 in the central nervous system areas suggested from the single-cell data.Conclusions Glra3 expression is low but widespread in the mouse central nervous system. Clear sex-dependent differences have been identified, indicating higher levels of Glra3 in several telencephalic and diencephalic areas, as well as in cerebellum and brainstem, in male mice compared with female mice.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Nyckelord
- Glycine
- Glra3
- brain
- spinal cord
- mice
- sex-dependent differences
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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