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Glioblastoma stem c...
Glioblastoma stem cells express non-canonical proteins and exclusive mesenchymal-like or non-mesenchymal-like protein signatures
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- Babacic, Haris (author)
- Karolinska Institutet,Karolinska Inst, Dept Oncol & Pathol, Sci Life Lab, Stockholm, Sweden.
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- Galardi, Silvia (author)
- Univ Roma Tor Vergata, Dept Biomed & Prevent, Rome, Italy.
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- Umer, Husen M. (author)
- Karolinska Inst, Dept Oncol & Pathol, Sci Life Lab, Stockholm, Sweden.
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- Hellström, Mats (author)
- Uppsala universitet,Cancerimmunterapi
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- Uhrbom, Lene (author)
- Uppsala universitet,Neuroonkologi och neurodegeneration
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- Maturi, Nagaprathyusha (author)
- Uppsala universitet,Neuroonkologi och neurodegeneration
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- Cardinali, Deborah (author)
- Univ Roma Tor Vergata, Dept Biomed & Prevent, Rome, Italy.
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- Pellegatta, Serena (author)
- Fdn IRCCS, Inst Neurol Carlo Besta, Dept Mol Neurooncol, Unit Immunotherapy Brain Tumors, Milan, Italy.
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- Michienzi, Alessandro (author)
- Univ Roma Tor Vergata, Dept Biomed & Prevent, Rome, Italy.
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- Trevisi, Gianluca (author)
- G D Annunzio Univ, Hosp Spirito St, Neurosurg Unit, Chieti, Pescara, Italy.
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- Mangiola, Annunziato (author)
- G D Annunzio Univ, Hosp Spirito St, Neurosurg Unit, Chieti, Pescara, Italy.
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- Lehtiö, Janne (author)
- Karolinska Institutet,Karolinska Inst, Dept Oncol & Pathol, Sci Life Lab, Stockholm, Sweden.
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- Ciafré, Silvia Anna (author)
- Univ Roma Tor Vergata, Dept Biomed & Prevent, Rome, Italy.;Univ Rome TorVergata, Dept Biomedicineand Prevent, Via Montpellier 1, I-00133 Rome, Italy.
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- Pernemalm, Maria (author)
- Karolinska Institutet,Karolinska Inst, Dept Oncol & Pathol, Sci Life Lab, Stockholm, Sweden.;Karolinska Inst, Sci Life Lab, Tomtebodavagen 23, S-17165 Stockholm, Sweden.
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Karolinska Institutet Karolinska Inst, Dept Oncol & Pathol, Sci Life Lab, Stockholm, Sweden (creator_code:org_t)
- 2023-01-16
- 2023
- English.
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In: Molecular Oncology. - : John Wiley & Sons. - 1574-7891 .- 1878-0261. ; 17:2, s. 238-260
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Abstract
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- Glioblastoma (GBM) cancer stem cells (GSCs) contribute to GBM's origin, recurrence, and resistance to treatment. However, the understanding of how mRNA expression patterns of GBM subtypes are reflected at global proteome level in GSCs is limited. To characterize protein expression in GSCs, we performed in-depth proteogenomic analysis of patient-derived GSCs by RNA-sequencing and mass-spectrometry. We quantified > 10 000 proteins in two independent GSC panels and propose a GSC-associated proteomic signature characterizing two distinct phenotypic conditions; one defined by proteins upregulated in proneural and classical GSCs (GPC-like), and another by proteins upregulated in mesenchymal GSCs (GM-like). The GM-like protein set in GBM tissue was associated with necrosis, recurrence, and worse overall survival. Through proteogenomics, we discovered 252 non-canonical peptides in the GSCs, i.e., protein sequences that are variant or derive from genome regions previously considered non-protein-coding, including variants of the heterogeneous ribonucleoproteins implicated in RNA splicing. In summary, GSCs express two protein sets that have an inverse association with clinical outcomes in GBM. The discovery of non-canonical protein sequences questions existing gene models and pinpoints new protein targets for research in GBM.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Keyword
- glioblastoma stem cells
- mesenchymal
- proneural
- proteogenomics
- proteomics
- signature
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Babacic, Haris
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Galardi, Silvia
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Umer, Husen M.
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Hellström, Mats
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Uhrbom, Lene
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Maturi, Nagaprat ...
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Cardinali, Debor ...
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Pellegatta, Sere ...
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Michienzi, Aless ...
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Trevisi, Gianluc ...
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Mangiola, Annunz ...
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Lehtiö, Janne
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Ciafré, Silvia A ...
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Pernemalm, Maria
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Cell and Molecul ...
- Articles in the publication
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Molecular Oncolo ...
- By the university
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Uppsala University
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Karolinska Institutet