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Dynamics of release...
Dynamics of release factor recycling during translation termination in bacteria
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- Prabhakar, Arjun (författare)
- Stanford Univ, Dept Struct Biol, Sch Med, Stanford, CA 94305 USA.;Stanford Univ, Program Biophys, Stanford, CA USA.;Pacific Biosci Inc, Menlo Pk, CA 94025 USA
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- Pavlov, Michael Y. (författare)
- Uppsala universitet,Molekylärbiologi
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- Zhang, Jingji (författare)
- Stanford Univ, Dept Struct Biol, Sch Med, Stanford, CA 94305 USA
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- Indrisiunaite, Gabriele (författare)
- Uppsala universitet,Molekylärbiologi
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- Wang, Jinfan (författare)
- Stanford Univ, Dept Struct Biol, Sch Med, Stanford, CA 94305 USA
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- Lawson, Michael R. (författare)
- Stanford Univ, Dept Struct Biol, Sch Med, Stanford, CA 94305 USA
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- Ehrenberg, Måns (författare)
- Uppsala universitet,Molekylärbiologi
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- Puglisi, Joseph D. (författare)
- Stanford Univ, Dept Struct Biol, Sch Med, Stanford, CA 94305 USA
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Stanford Univ, Dept Struct Biol, Sch Med, Stanford, CA 94305 USA;Stanford Univ, Program Biophys, Stanford, CA USA.;Pacific Biosci Inc, Menlo Pk, CA 94025 USA Molekylärbiologi (creator_code:org_t)
- Oxford University Press, 2023
- 2023
- Engelska.
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Ingår i: Nucleic Acids Research. - : Oxford University Press. - 0305-1048 .- 1362-4962. ; 51:11, s. 5774-5790
- Relaterad länk:
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https://doi.org/10.1...
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https://uu.diva-port... (primary) (Raw object)
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- In bacteria, release of newly synthesized proteins from ribosomes during translation termination is catalyzed by class-I release factors (RFs) RF1 or RF2, reading UAA and UAG or UAA and UGA codons, respectively. Class-I RFs are recycled from the post-termination ribosome by a class-II RF, the GTPase RF3, which accelerates ribosome intersubunit rotation and class-I RF dissociation. How conformational states of the ribosome are coupled to the binding and dissociation of the RFs remains unclear and the importance of ribosome-catalyzed guanine nucleotide exchange on RF3 for RF3 recycling in vivo has been disputed. Here, we profile these molecular events using a single-molecule fluorescence assay to clarify the timings of RF3 binding and ribosome intersubunit rotation that trigger class-I RF dissociation, GTP hydrolysis, and RF3 dissociation. These findings in conjunction with quantitative modeling of intracellular termination flows reveal rapid ribosome-dependent guanine nucleotide exchange to be crucial for RF3 action in vivo.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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