Search: onr:"swepub:oai:DiVA.org:uu-62803" >
A comprehensive pol...
A comprehensive polymerase chain reaction-oligonucleotide typing system for the HLA class I A locus
-
- Allen, Marie (author)
- Uppsala universitet,Institutionen för genetik och patologi
-
Liu, Limin (author)
-
- Gyllensten, Ulf (author)
- Uppsala universitet,Institutionen för genetik och patologi
-
(creator_code:org_t)
- Elsevier BV, 1994
- 1994
- English.
-
In: Human Immunology. - : Elsevier BV. - 0198-8859 .- 1879-1166. ; 40:1, s. 25-32
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
show less...
Abstract
Subject headings
Close
- A comprehensive system for genetic typing of the HLA class I A locus is described, based on PCR amplification and typing with nonradioactively labeled SSO probes. Exons 1-3 of the A locus are amplified and typing is performed with a set of 30 nonradioactively labeled oligonucleotide probes. This system resolves 34 of 39 known alleles and 561 (94%) of 595 possible genotypes. Among a sample of 354 individuals from Sweden and China, 97.5% of the genotypes were resolved. Probes were directed preferentially at replacement substitutions in foreign antigen-binding sites, in order to detect not only the known alleles but also new combinations of polymorphic motifs, indicative of previously unrecognized alleles. Three individuals were found with a new combination of polymorphic motifs, suggesting the presence of at least one previously undescribed allele in the populations sampled. This typing system is useful for disease association studies, tissue typing, and in forensic medicine.
Keyword
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database