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An optimistic view ...
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Preece, Paul
(författare)
An optimistic view for quantifying mRNA in post-mortem human brain
- Artikel/kapitelEngelska2003
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LIBRIS-ID:oai:DiVA.org:uu-72472
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-72472URI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:ref swepub-contenttype
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Ämneskategori:art swepub-publicationtype
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Quantitative human mRNA data are derived from post-mortem or biopsied tissue. RNA degradation, poor replication, a large mRNA variance and confounding factors such as brain pH and age of death are often cited, however, as objections to the data's reliability. A central question is whether post-mortem human mRNA can be treated as a statistically ordered system. TaqMan real-time RT-PCR was used to measure seven mRNAs in 513 cortical samples taken from 90 Alzheimer's disease and 81 control brains. Despite a high mRNA variance strong correlations were found between the mRNA transcripts in a single brain. Where a brain has a high/low level of one mRNA, the same brain invariably has a high/low level of other mRNAs; correlated order is present and allows removal of that source of variation common to all genes. Although levels of mRNA are highly variable between subjects (>1000-fold), quantitative order is present in post-mortem human mRNA, allowing effects due to pathology or gender to be isolated and tested for significance.
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Biuppslag (personer, institutioner, konferenser, titlar ...)
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Virley, David J
(författare)
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Costandi, Moheb
(författare)
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Coombes, Robert
(författare)
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Moss, Stephen J
(författare)
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Mudge, Anne W
(författare)
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Jazin, ElenaUppsala universitet,Evolutionsbiologi,Behavioral Genetics(Swepub:uu)elenjazi
(författare)
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Cairns, Nigel J
(författare)
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Uppsala universitetEvolutionsbiologi
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:Brain Research. Molecular Brain Research116:1-2, s. 7-160169-328X1872-6941
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