Sökning: onr:"swepub:oai:DiVA.org:uu-83489" >
Loss of T-Cell Prot...
Loss of T-Cell Protein Tyrosine Phosphatase Induces Recycling of the Platelet-derived Growth Factor (PDGF) beta-Receptor but Not the PDGF {alpha}-Receptor
-
- Karlsson, Susann (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
-
- Kowanetz, Katarzyna (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
-
- Sandin, Åsa (författare)
- Karolinska Institutet
-
visa fler...
-
- Persson, Camilla (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
-
- Östman, Arne (författare)
- Karolinska Institutet
-
- Heldin, Carl-Henrik (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
-
- Hellberg, Carina (författare)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
-
visa färre...
-
(creator_code:org_t)
- 2006
- 2006
- Engelska.
-
Ingår i: Molecular Biology of the Cell. - 1059-1524 .- 1939-4586. ; 17:11, s. 4846-4855
- Relaterad länk:
-
https://urn.kb.se/re...
-
visa fler...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- We have previously shown that the T-cell protein tyrosine phosphatase (TC-PTP) dephosphorylates the platelet-derived growth factor (PDGF) beta-receptor. Here, we show that the increased PDGF beta-receptor phosphorylation in TC-PTP knockout (ko) mouse embryonic fibroblasts (MEFs) occurs primarily on the cell surface. The increased phosphorylation is accompanied by a TC-PTP-dependent, monensin-sensitive delay in clearance of cell surface PDGF beta-receptors and delayed receptor degradation, suggesting PDGF beta-receptor recycling. Recycled receptors could also be directly detected on the cell surface of TC-PTP ko MEFs. The effect of TC-PTP depletion was specific for the PDGF beta-receptor, because PDGF alpha-receptor homodimers were cleared from the cell surface at the same rate in TC-PTP ko MEFs as in wild-type MEFs. Interestingly, PDGF alphabeta-receptor heterodimers were recycling. Analysis by confocal microscopy revealed that, in TC-PTP ko MEFs, activated PDGF beta-receptors colocalized with Rab4a, a marker for rapid recycling. In accordance with this, transient expression of a dominant-negative Rab4a construct increased the rate of clearance of cell surface receptors on TC-PTP ko MEFs. Thus, loss of TC-PTP specifically redirects the PDGF beta-receptor toward rapid recycling, which is the first evidence of differential trafficking of PDGF receptor family members.
Nyckelord
- MEDICINE
- MEDICIN
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas