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Digital Quantificat...
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Jarvius, JonasUppsala universitet,Institutionen för genetik och patologi
(author)
Digital Quantification using Amplified Single-Molecule Detection
- Article/chapterEnglish2006
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Numbers
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LIBRIS-ID:oai:DiVA.org:uu-95118
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-95118URI
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https://doi.org/10.1038/NMETH916DOI
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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We describe a scheme for biomolecule enumeration by converting nanometer-scale specific molecular recognition events mediated by rolling-circle amplification to fluorescent micrometer-sized DNA molecules amenable to discrete optical detection. Our amplified single-molecule detection (SMD) approach preserves the discrete nature of the molecular population, allowing multiplex detection and highly precise quantification of molecules over a dynamic range of seven orders of magnitude. We apply the method for sensitive detection and quantification of the bacterial pathogen Vibrio cholerae.
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Melin, JonasUppsala universitet,Fasta tillståndets fysik(Swepub:uu)jomel499
(author)
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Göransson, JennyUppsala universitet,Institutionen för genetik och patologi(Swepub:uu)jegor108
(author)
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Stenberg, JohanUppsala universitet,Institutionen för genetik och patologi(Swepub:uu)joste021
(author)
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Fredriksson, SimonUppsala universitet,Institutionen för genetik och patologi
(author)
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Gonzalez-Rey, CarlosUppsala universitet,Limnologi,Mikrobiell Ekologi
(author)
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Bertilsson, StefanUppsala universitet,Limnologi,Mikrobiell Ekologi(Swepub:uu)stber839
(author)
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Nilsson, MatsUppsala universitet,Institutionen för genetik och patologi(Swepub:uu)matsnils
(author)
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Uppsala universitetInstitutionen för genetik och patologi
(creator_code:org_t)
Related titles
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In:Nature Methods3:9, s. 725-7271548-70911548-7105
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