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Monocytes and macro...
Monocytes and macrophages, implications for breast cancer migration and stem cell-like activity and treatment
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Ward, R. (author)
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Sims, A. H. (author)
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Lee, A. (author)
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Lo, C. (author)
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Wynne, L. (author)
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Yusuf, H. (author)
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Gregson, H. (author)
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Lisanti, M. p (author)
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Sotgia, F. (author)
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- Landberg, Göran, 1963 (author)
- Gothenburg University,Göteborgs universitet,Sahlgrenska Cancer Center
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Lamb, R. (author)
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(creator_code:org_t)
- 2015
- 2015
- English.
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In: OncoTarget. - 1949-2553. ; 6:16, s. 14687-14699
- Related links:
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https://gup.ub.gu.se...
Abstract
Subject headings
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- Macrophages are a major cellular constituent of the tumour stroma and contribute to breast cancer prognosis. The precise role and treatment strategies to target macrophages remain elusive. As macrophage infiltration is associated with poor prognosis and high grade tumours we used the THP-1 cell line to model monocytemacrophage differentiation in co-culture with four breast cancer cell lines (MCF7, T47D, MDA-MB-231, MDA-MB-468) to model in vivo cellular interactions. Polarisation into M1 and M2 subtypes was confirmed by specific cell marker expression of ROS and HLA-DR, respectively. Co-culture with all types of macrophage increased migration of ER-positive breast cancer cell lines, while M2-macrophages increased mammosphere formation, compared to M1-macrophages, in all breast cancer cells lines. Treatment of cells with Zoledronate in co-culture reduced the "pro-tumourigenic" effects (increased mammospheres/migration) exerted by macrophages. Direct treatment of breast cancer cells in homotypic culture was unable to reduce migration or mammosphere formation. Macrophages promote "pro-tumourigenic" cellular characteristics of breast cancer cell migration and stem cell activity. Zoledronate targets macrophages within the microenvironment which in turn, reduces the "pro-tumourigenic" characteristics of breast cancer cells. Zoledronate offers an exciting new treatment strategy for both primary and metastatic breast cancer.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- Breast
- Macrophage
- Migration
- Stem-cells
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Ward, R.
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Sims, A. H.
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Lee, A.
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Lo, C.
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Wynne, L.
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Yusuf, H.
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show more...
-
Gregson, H.
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Lisanti, M. p
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Sotgia, F.
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Landberg, Göran, ...
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Lamb, R.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Cancer and Oncol ...
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OncoTarget
- By the university
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University of Gothenburg