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The role of the G p...
The role of the G protein-coupled receptor GPR30 in the effects of estrogen in ovariectomized mice.
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- Windahl, Sara H, 1971 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin,Institute of Medicine, Department of Internal Medicine,University of Gothenburg
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- Andersson, Niklas, 1970 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology,University of Gothenburg
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- Chagin, A S (author)
- Karolinska Institutet
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- Mårtensson, Ulrika (author)
- Lund University,Lunds universitet,Drug Target Discovery,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Carlsten, Hans, 1954 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research
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- Olde, Björn (author)
- Lund University,Lunds universitet,Drug Target Discovery,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Swanson, Charlotte, 1975 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin,Institute of Medicine, Department of Internal Medicine,University of Gothenburg
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- Movérare-Skrtic, Sofia (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin,Institute of Medicine, Department of Internal Medicine,University of Gothenburg
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- Sävendahl, L (author)
- Karolinska Institutet
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- Lagerquist, Marie (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Internal Medicine,Institute of Medicine, Department of Rheumatology and Inflammation Research
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- Leeb-Lundberg, Fredrik (author)
- Lund University,Lunds universitet,Drug Target Discovery,Forskargrupper vid Lunds universitet,Lund University Research Groups
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- Ohlsson, Claes, 1965 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin,Institute of Medicine, Department of Internal Medicine,University of Gothenburg
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- Lindholm, Catharina, 1967 (author)
- Göteborgs universitet,University of Gothenburg
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- Fick, Jerker (author)
- Göteborgs universitet,University of Gothenburg
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(creator_code:org_t)
- American Physiological Society, 2009
- 2009
- English.
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In: American journal of physiology. Endocrinology and metabolism. - : American Physiological Society. - 0193-1849 .- 1522-1555. ; 296:3
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Abstract
Subject headings
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- In vitro studies suggest that the membrane G protein-coupled receptor GPR30 is a functional estrogen receptor (ER). The aim of the present study was to determine the possible in vivo role of GPR30 as a functional ER primarily for the regulation of skeletal parameters, including bone mass and longitudinal bone growth, but also for some other well-known estrogen-regulated parameters, including uterine weight, thymus weight, and fat mass. Three-month-old ovariectomized (OVX) GPR30-deficient mice (GPR30(-/-)) and wild-type (WT) mice were treated with either vehicle or increasing doses of estradiol (E(2); 0, 30, 70, 160, or 830 ng.mouse(-1).day(-1)). Body composition [bone mineral density (BMD), fat mass, and lean mass] was analyzed by dual-energy-X ray absorptiometry, while the cortical and trabecular bone compartments were analyzed by peripheral quantitative computerized tomography. Quantitative histological analyses were performed in the distal femur growth plate. Bone marrow cellularity and distribution were analyzed using a fluorescence-activated cell sorter. The estrogenic responses on most of the investigated parameters, including increase in bone mass (total body BMD, spine BMD, trabecular BMD, and cortical bone thickness), increase in uterine weight, thymic atrophy, fat mass reduction, and increase in bone marrow cellularity, were similar for all of the investigated E(2) doses in WT and GPR30(-/-) mice. On the other hand, E(2) treatment reduced longitudinal bone growth, reflected by decreased femur length and distal femur growth plate height, in the WT mice but not in the GPR30(-/-) mice compared with vehicle-treated mice. These in vivo findings demonstrate that GPR30 is not required for normal estrogenic responses on several major well-known estrogen-regulated parameters. In contrast, GPR30 is required for a normal estrogenic response in the growth plate.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Keyword
- Adipose Tissue
- anatomy & histology
- growth & development
- Animals
- Bone Density
- Bone Development
- physiology
- Estrogens
- metabolism
- Female
- Femur
- cytology
- growth & development
- Growth Plate
- cytology
- growth & development
- Mice
- Mice
- Mutant Strains
- Organ Size
- Ovariectomy
- Receptors
- Estrogen
- metabolism
- Receptors
- G-Protein-Coupled
- genetics
- metabolism
- Thymus Gland
- anatomy & histology
- growth & development
- Uterus
- anatomy & histology
- growth & development
- Estrogen
- bone
- estrogen receptor
- growth
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Windahl, Sara H, ...
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Andersson, Nikla ...
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Chagin, A S
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Mårtensson, Ulri ...
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Carlsten, Hans, ...
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Olde, Björn
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Swanson, Charlot ...
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Movérare-Skrtic, ...
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Sävendahl, L
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Lagerquist, Mari ...
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Leeb-Lundberg, F ...
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Ohlsson, Claes, ...
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Lindholm, Cathar ...
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Fick, Jerker
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Endocrinology an ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Physiology
- Articles in the publication
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American journal ...
- By the university
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University of Gothenburg
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Chalmers University of Technology
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Lund University
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Karolinska Institutet