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Delta-opioid recept...
Delta-opioid receptors on astroglial cells in primary culture: mobilization of intracellular free calcium via a pertussis sensitive G protein.
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- Thorlin, Thorleif, 1964 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap,Institute of Clinical Neurosciences
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- Eriksson, Peter S, 1959 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap, Sektionen för kliniska nervsjukdomar,Institute of Clinical Neurosciences, Section of Neurological Diseases
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- Persson, Anders I., 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap,Institute of Clinical Neurosciences
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- Åberg, N David, 1970 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap, Sektionen för kliniska nervsjukdomar,Institute of Clinical Neurosciences, Section of Neurological Diseases
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- Hansson, Elisabeth, 1955 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap,Institute of Clinical Neurosciences
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- Rönnbäck, Lars, 1951 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap,Institute of Clinical Neurosciences
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(creator_code:org_t)
- 1998
- 1998
- English.
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In: Neuropharmacology. - 0028-3908. ; 37:3, s. 299-311
- Related links:
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https://gup.ub.gu.se...
Abstract
Subject headings
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- Astrocytes in primary culture from rat cerebral cortex were probed concerning the expression of delta-opioid receptors and their coupling to changes in intracellular free calcium concentrations ([Ca2+]i). Fluo-3 or fura-2 based microspectrofluorometry was used for [Ca2+]i measurements on single astrocytes in a mixed astroglial-neuronal culture. Application of the selective delta-opioid receptor agonist, [D-Pen2, D-Pen5]-enkephalin (DPDPE), at concentrations ranging from 10 nM to 100 microM, induced concentration-dependent increases in [Ca2+]i (EC50 = 114 nM). The responses could be divided into two phases, with an initial spike in [Ca2+]i followed by either oscillations or a sustained elevation of [Ca2+]i. These effects were blocked by the selective delta-opioid receptor antagonist ICI 174864 (10 microM). The expression of delta-opioid receptors on astroglial cells was further verified immunohistochemically, using specific antibodies, and by Western blot analyses. Pre-treatment of the cells with pertussis toxin (100 ng/ml, 24 h) blocked the effects of delta-opioid receptor activation, consistent with a Gi- or Go-mediated response. The sustained elevation of [Ca2+]i was not observed in low extracellular Ca2+ and was partly blocked by nifedipine (1 microM), indicating the involvement of L-type Ca2+ channels. Stimulating neurons with DPDPE resulted in a decrease in [Ca2+]i, which may be consistent with the closure of the plasma membrane Ca2+ channels on these cells. The current results suggest a role for astrocytes in the response of the brain to delta-opioid peptides and that these opioid effects in part involve altered astrocytic intracellular Ca2+ homeostasis.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Keyword
- Animals
- Astrocytes
- drug effects
- metabolism
- Calcium
- metabolism
- Calcium Channels
- metabolism
- Cells
- Cultured
- Cerebral Cortex
- drug effects
- metabolism
- Enkephalin
- D-Penicillamine (2
- 5)-
- Enkephalins
- pharmacology
- GTP-Binding Proteins
- antagonists & inhibitors
- metabolism
- Immunohistochemistry
- Neuroglia
- drug effects
- metabolism
- Neurons
- drug effects
- metabolism
- Pertussis Toxin
- Rats
- Rats
- Sprague-Dawley
- Receptors
- Opioid
- delta
- agonists
- physiology
- Virulence Factors
- Bordetella
- pharmacology
Publication and Content Type
- ref (subject category)
- art (subject category)
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