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Detection of surfac...
Detection of surface bound complement at increasing serum anticoagulant concentrations.
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- Arvidsson, Sara, 1977- (author)
- Linköpings universitet,Tekniska högskolan,Tillämpad Fysik
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- Askendal, Agneta, 1945- (author)
- Linköpings universitet,Tekniska högskolan,Tillämpad Fysik
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- Lindahl, Tomas, 1954- (author)
- Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Klinisk kemi
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- Tengvall, Pentti (author)
- Linköpings universitet,Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för biomaterialvetenskap,Institute of Clinical Sciences, Department of Biomaterials,Tekniska högskolan,Tillämpad Fysik
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(creator_code:org_t)
- Elsevier BV, 2008
- 2008
- English.
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In: Colloids and surfaces. B, Biointerfaces. - : Elsevier BV. - 0927-7765 .- 1873-4367. ; 62:2, s. 214-9
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Abstract
Subject headings
Close
- Surface mediated immune complement activation can be detected by a variety of antibody utilizing methods such as ELISA, fluorescence- or radiolabelling techniques, QCM, and ellipsometry. In the present work we investigated how the common anticoagulants heparin, dalteparin, fondaparinux and sodium citrate affected the binding of anti-complement factor 3c (anti-C3c) on a model complement activator surface, immobilised IgG, after incubation in human blood serum. The results show, as expected, that different anticoagulants affect the antibody binding differently. Increasing amounts of heparin, dalteparin and sodium citrate in normal serum resulted in a decreasing anti-C3c binding. The antibody deposition was not sensitive for the fondaparinux concentration. Surprisingly high concentrations of anti-coagulantia were needed to completely eradicate the antibody binding. Experiments in EGTA-serum showed that anticoagulants interfered directly with both the classical and alternative pathways. Control C3a-des arg ELISA measurements show that the lowered antibody surface binding was not a result of complement depletion in serum. Kallikrein generation by hydrophilic glass surfaces was not affected by high anticoagulant concentrations.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kirurgi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Surgery (hsv//eng)
Keyword
- Anticoagulants
- blood
- pharmacology
- Blood Proteins
- metabolism
- Colorimetry
- Complement Activation
- drug effects
- Complement C3
- metabolism
- Complement C3a
- metabolism
- Complement System Proteins
- analysis
- Dalteparin
- pharmacology
- Egtazic Acid
- diagnostic use
- Enzyme-Linked Immunosorbent Assay
- Heparin
- pharmacology
- Humans
- Immunoassay
- Immunoglobulin G
- chemistry
- Indicators and Reagents
- Kallikreins
- Molecular Weight
- Polysaccharides
- pharmacology
- Surface Plasmon Resonance
- Surface Properties
- NATURAL SCIENCES
Publication and Content Type
- ref (subject category)
- art (subject category)
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