Sökning: onr:"swepub:oai:gup.ub.gu.se/165196" >
Protein sliding and...
Protein sliding and DNA denaturation are essential for DNA organization by human mitochondrial transcription factor A
-
Farge, G. (författare)
-
Laurens, N. (författare)
-
Broekmans, O. D. (författare)
-
visa fler...
-
van den Wildenberg, Smjl (författare)
-
Dekker, L. C. M. (författare)
-
Gaspari, M. (författare)
-
- Gustafsson, Claes M, 1966 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
-
Peterman, E. J. G. (författare)
-
- Falkenberg, Maria, 1968 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för medicinsk kemi och cellbiologi,Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology
-
Wuite, G. J. L. (författare)
-
visa färre...
-
(creator_code:org_t)
- 2012-08-21
- 2012
- Engelska.
-
Ingår i: Nature Communications. - : Springer Science and Business Media LLC. - 2041-1723. ; 3
- Relaterad länk:
-
https://www.nature.c...
-
visa fler...
-
https://gup.ub.gu.se...
-
https://doi.org/10.1...
-
http://kipublication...
-
visa färre...
Abstract
Ämnesord
Stäng
- Mitochondria organize their genome in protein-DNA complexes called nucleoids. The mitochondrial transcription factor A (TFAM), a protein that regulates mitochondrial transcription, is abundant in these nucleoids. TFAM is believed to be essential for mitochondrial DNA compaction, yet the exact mechanism has not been resolved. Here we use a combination of single-molecule manipulation and fluorescence microscopy to show the nonspecific DNA-binding dynamics and compaction by TFAM. We observe that single TFAM proteins diffuse extensively over DNA (sliding) and, by collisions, form patches on DNA in a cooperative manner. Moreover, we demonstrate that TFAM induces compaction by changing the flexibility of the DNA, which can be explained by local denaturation of the DNA (melting). Both sliding of TFAM and DNA melting are also necessary characteristics for effective, specific transcription regulation by TFAM. This apparent connection between transcription and DNA organization clarifies how TFAM can accomplish two complementary roles in the mitochondrial nucleoid at the same time.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology (hsv//eng)
Nyckelord
- factor-a
- bacterial chromatin
- architectural role
- optical tweezers
- binding
- tension
- microscopy
- nucleoids
- diffusion
- reveals
- ghee jd
- 1974
- journal of molecular biology
- v86
- p469
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
Hitta via bibliotek
Till lärosätets databas