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Variability of the ...
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Rekabdar, Elham,1971Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk virologi,Institute of Laboratory Medicine, Dept of Clinical Virology
(author)
Variability of the glycoprotein G gene in clinical isolates of herpes simplex virus type 1.
- Article/chapterEnglish1999
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Numbers
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LIBRIS-ID:oai:gup.ub.gu.se/179829
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https://gup.ub.gu.se/publication/179829URI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Glycoprotein G (gG) of herpes simplex virus type 1 (HSV-1) has been used as a prototype antigen for HSV-1 type-specific serodiagnosis, but data on the sequence variability of the gene coding for this protein in wild-type strains are lacking. In this study, direct DNA sequencing of the gG-1 genes from PCR products was performed with clinical HSV-1 isolates from 11 subjects as well as with strains Syn 17(+), F, and KOS 321. The reference strains Syn 17(+) and F showed a high degree of conservation, while KOS 321 carried 13 missense mutations and, in addition, 12 silent mutations. Three clinical isolates showed mutations leading to amino acid alterations: one had a mutation of K(122) to N, which is a gG-1-to-gG-2 alteration; another contained all mutations which were observed in KOS 321 except two silent mutations; and the third isolate carried five missense mutations. Two clinical isolates as well as strain KOS 321 showed a mutation (F(111)-->V) within the epitope of a gG-1-reactive monoclonal antibody (MAb). When all viruses were tested for reactivity with the anti-gG-1 MAb, the three strains with the F(111)-->V mutation were found to be unreactive. Furthermore, gG-1 antibodies purified from sera from the two patients carrying strains mutated in this epitope were less reactive when they were tested by an HSV-1-infected-cell assay. Therefore, our finding that the sequence variability of the gG-1 gene also affects B-cell epitope regions of this protein in clinical isolates may have consequences for the use of this protein as a type-specific antigen for serodiagnosis.
Subject headings and genre
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MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Dermatologi och venereologi hsv//swe
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MEDICAL AND HEALTH SCIENCES Clinical Medicine Dermatology and Venereal Diseases hsv//eng
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Adult
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Aged
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Amino Acid Sequence
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Amino Acid Substitution
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Animals
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Antibodies
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Monoclonal
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Antibodies
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Viral
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immunology
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Antigens
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Surface
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analysis
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immunology
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Cells
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Cultured
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Cercopithecus aethiops
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DNA Mutational Analysis
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DNA
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Viral
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analysis
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Enzyme-Linked Immunosorbent Assay
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Epitopes
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immunology
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Female
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Genetic Variation
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Herpes Simplex
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genetics
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immunology
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Herpesvirus 1
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Human
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genetics
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immunology
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Humans
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Immunoglobulin G
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immunology
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Kidney
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cytology
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Male
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Middle Aged
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Molecular Sequence Data
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Polymerase Chain Reaction
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Sensitivity and Specificity
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Serologic Tests
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Viral Envelope Proteins
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genetics
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immunology
Added entries (persons, corporate bodies, meetings, titles ...)
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Tunbäck, Petra,1965Gothenburg University,Göteborgs universitet,Institutionen för särskilda specialiteter, Avdelningen för dermatologi och venereologi,Institute of Selected Clinical Sciences, Department of Dermatology and Venereology(Swepub:gu)xtunpe
(author)
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Liljeqvist, Jan-Åke,1954Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk virologi,Institute of Laboratory Medicine, Dept of Clinical Virology(Swepub:gu)xljanl
(author)
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Bergström, Tomas,1950Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk virologi,Institute of Laboratory Medicine, Dept of Clinical Virology(Swepub:gu)xberto
(author)
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Göteborgs universitetInstitutionen för laboratoriemedicin, Avdelningen för klinisk virologi
(creator_code:org_t)
Related titles
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In:Clinical and diagnostic laboratory immunology6:6, s. 826-311071-412X
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