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The mucosal adjuvan...
The mucosal adjuvant effects of cholera toxin and immune-stimulating complexes differ in their requirement for IL-12, indicating different pathways of action.
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- Grdic Eliasson, Dubravka (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk mikrobiologi och immunologi,Institute of Medical Microbiology/Immunology
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Smith, R (författare)
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Donachie, A (författare)
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visa fler...
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Kjerrulf, Martin (författare)
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- Hultgren-Hörnquist, Elisabeth, 1965 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk immunologi,Institute of Laboratory Medicine, Dept of Clinical Immunology
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Mowat, A (författare)
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- Lycke, Nils Y, 1954 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk mikrobiologi och immunologi,Institute of Medical Microbiology/Immunology
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visa färre...
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(creator_code:org_t)
- 1999
- 1999
- Engelska.
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Ingår i: European journal of immunology. - 0014-2980. ; 29:6, s. 1774-84
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- Adjuvants that can improve mucosal vaccine efficacy are much warranted. In this comparative study between cholera toxin (CT) and immune-stimulating complexes (ISCOM) we found that, contrary to CT, ovalbumin (OVA)-ISCOM were poor inducers of mucosal anti-OVA IgA responses, but induced similar or better systemic immunity following oral immunizations. The addition of CT to the oral OVA-ISCOM protocol did not stimulate local anti-OVA IgA immunity, nor did it change the quality or magnitude of the systemic responses. Both vectors recruited strong innate immunity, but only OVA-ISCOM could directly induce IL-12, demonstrable at the protein and mRNA levels. CT had no inhibitory effects on lipopolysaccharide/IFN-gamma-induced IL-12 mRNA expression or IL-12 production. Furthermore, adjuvanticity of CT was unaffected in IL-12-deficient mice, while OVA-ISCOM showed partly impaired adjuvant effects by the lack of IL-12. CT abrogated the induction of oral tolerance stimulated by antigen feeding in these mice. In addition, CT did not alter TGF-beta levels, suggesting that the immunomodulating effect of CT was independent of IL-12 as well as TGF-beta production. Taken together, these findings indicate that mucosal adjuvanticity of CT and ISCOM are differently dependent on IL-12, suggesting that separate and distinct antigen-processing pathways are involved.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Nyckelord
- Adjuvants
- Immunologic
- administration & dosage
- Administration
- Oral
- Animals
- Base Sequence
- Cholera Toxin
- administration & dosage
- DNA Primers
- genetics
- ISCOMs
- administration & dosage
- Immune Tolerance
- Immunity
- Mucosal
- Immunoglobulin G
- blood
- Interleukin-12
- biosynthesis
- genetics
- Lymphocyte Activation
- Mice
- Mice
- Inbred BALB C
- Mice
- Inbred C57BL
- Mice
- Knockout
- Ovalbumin
- immunology
- RNA
- Messenger
- genetics
- metabolism
- T-Lymphocytes
- immunology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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