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The regulation of n...
The regulation of nitric oxide synthase isoform expression in mouse and human fallopian tubes: Potential insights for ectopic pregnancy
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- Hu, Junting (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology
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Ma, S. (author)
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Zou, S. (author)
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Li, X. (author)
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Cui, P. (author)
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- Weijdegård, Birgitta (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology
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Wu, G. (author)
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- Shao, Linus Ruijin, 1964 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology
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- Billig, Håkan, 1955 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology
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- Feng, Yi (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för fysiologi,Institute of Neuroscience and Physiology, Department of Physiology
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(creator_code:org_t)
- 2014-12-23
- 2015
- English.
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In: International Journal of Molecular Sciences. - : MDPI AG. - 1422-0067. ; 16:1, s. 49-67
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Abstract
Subject headings
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- Nitric oxide (NO) is highly unstable and has a half-life of seconds in buffer solutions. It is synthesized by NO-synthase (NOS), which has been found to exist in the following three isoforms: neuro nitric oxide synthase (nNOS), inducible nitric oxide synthase (iNOS), and endothelial nitric oxide synthase (eNOS). NOS activity is localized in the reproductive tracts of many species, although direct evidence for NOS isoforms in the Fallopian tubes of mice is still lacking. In the present study, we investigated the expression and regulation of NOS isoforms in the mouse and human Fallopian tubes during the estrous and menstrual cycles, respectively. We also measured isoform expression in humans with ectopic pregnancy and in mice treated with lipopolysaccharide (LPS). Our results confirmed the presence of different NOS isoforms in the mouse and human Fallopian tubes during different stages of the estrous and menstrual cycles and showed that iNOS expression increased in the Fallopian tubes of women with ectopic pregnancy and in LPS-treated mice. Elevated iNOS activity might influence ovulation, cilia beats, contractility, and embryo transportation in such a manner as to increase the risk of ectopic pregnancy. This study has provided morphological and molecular evidence that NOS isoforms are present and active in the human and mouse Fallopian tubes and suggests that iNOS might play an important role in both the reproductive cycle and infection-induced ectopic pregnancies.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Reproduktionsmedicin och gynekologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Obstetrics, Gynaecology and Reproductive Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Keyword
- Ectopic pregnancy
- Fallopian tube
- Nitric oxide synthase
- Steroid hormones
- endothelial nitric oxide synthase
- estradiol
- inducible nitric oxide synthase
- lactate dehydrogenase
- luteinizing hormone
- messenger RNA
- neuronal nitric oxide synthase
- progesterone
- adult
- animal experiment
- animal tissue
- Article
- controlled study
- enzyme activity
- female
- human
- human tissue
- immunofluorescence
- immunohistochemistry
- menstrual cycle
- mouse
- muscle contractility
- nonhuman
- ovulation
- protein expression
- real time polymerase chain reaction
- regulatory mechanism
- reverse transcription polymerase chain reaction
- RNA extraction
- uterine tube
- Mus
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Hu, Junting
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Ma, S.
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Zou, S.
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Li, X.
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Cui, P.
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Weijdegård, Birg ...
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show more...
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Wu, G.
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Shao, Linus Ruij ...
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Billig, Håkan, 1 ...
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Feng, Yi
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Obstetrics Gynae ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Physiology
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International Jo ...
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University of Gothenburg