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The resistomes of s...
The resistomes of six carbapenem-resistant pathogens - a critical genotype-phenotype analysis
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- Johnning, Anna, 1985 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för matematiska vetenskaper,CARe - Centrum för antibiotikaresistensforskning,Department of Mathematical Sciences,Centre for antibiotic resistance research, CARe,University of Gothenburg,Chalmers tekniska högskola,Chalmers University of Technology
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- Karami, Nahid, 1959 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,CARe - Centrum för antibiotikaresistensforskning,Institute of Biomedicine, Department of Infectious Medicine,Centre for antibiotic resistance research, CARe,University of Gothenburg
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- Tång Hallbäck, Erika, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine,University of Gothenburg
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- Müller, Vilhelm, 1990 (author)
- Chalmers University of Technology
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- Nyberg, Lena, 1979 (author)
- Chalmers University of Technology
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- Buongermino Pereira, Mariana, 1982 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,CARe - Centrum för antibiotikaresistensforskning,Institute of Biomedicine, Department of Infectious Medicine,Centre for antibiotic resistance research, CARe,University of Gothenburg,Chalmers tekniska högskola,Chalmers University of Technology
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- Stewart, C. (author)
- Lunds universitet,Lund University
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- Ambjörnsson, Tobias, 1974 (author)
- Lund University,Lunds universitet,Beräkningsbiologi och biologisk fysik - Har omorganiserats,Institutionen för astronomi och teoretisk fysik - Har omorganiserats,Naturvetenskapliga fakulteten,Computational Biology and Biological Physics - Has been reorganised,Department of Astronomy and Theoretical Physics - Has been reorganised,Faculty of Science
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- Westerlund, Fredrik, 1978 (author)
- Chalmers University of Technology
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- Adlerberth, Ingegerd, 1959 (author)
- Gothenburg University,Göteborgs universitet,CARe - Centrum för antibiotikaresistensforskning,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Centre for antibiotic resistance research, CARe,Institute of Biomedicine, Department of Infectious Medicine,University of Gothenburg
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- Kristiansson, Erik, 1978 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för matematiska vetenskaper,CARe - Centrum för antibiotikaresistensforskning,Department of Mathematical Sciences,Centre for antibiotic resistance research, CARe,University of Gothenburg,Chalmers tekniska högskola,Chalmers University of Technology
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(creator_code:org_t)
- Microbiology Society, 2018
- 2018
- English.
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In: Microbial Genomics. - : Microbiology Society. - 2057-5858. ; 4:11
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Abstract
Subject headings
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- Carbapenem resistance is a rapidly growing threat to our ability to treat refractory bacterial infections. To understand how carbapenem resistance is mobilized and spread between pathogens, it is important to study the genetic context of the underlying resistance mechanisms. In this study, the resistomes of six clinical carbapenem-resistant isolates of five different species - Acinetobacter baumannii, Escherichia colt, two Klebsiella pneumoniae, Proteus mirabilis and Pseudomonas aeruginosa - were characterized using whole genome sequencing. All Enterobacteriaceae isolates and the A. baumannii isolate had acquired a large number of antimicrobial resistance genes (7-18 different genes per isolate), including the following encoding carbapenemases: bla(KPC-2), bla(OXA-48), bla(OXA-72), bla(NDM-1), bla(NDm-7) and bla(VIM-1). In addition, a novel version of bla(SHv) was discovered. Four new resistance plasmids were identified and their fully assembled sequences were verified using optical DNA mapping. Most of the resistance genes were colocalized on these and other plasmids, suggesting a risk for coselection. In contrast, five out of six carbapenemase genes were present on plasmids with no or few other resistance genes. The expected level of resistance - based on acquired resistance determinants - was concordant with measured levels in most cases. There were, however, several important discrepancies for four of the six isolates concerning multiple classes of antibiotics. In conclusion, our results further elucidate the diversity of carbapenemases, their mechanisms of horizontal transfer and possible patterns of co-selection. The study also emphasizes the difficulty of using whole genome sequencing for antimicrobial susceptibility testing of pathogens with complex genotypes.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- NATURVETENSKAP -- Biologi -- Mikrobiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Microbiology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
- NATURVETENSKAP -- Fysik -- Annan fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Other Physics Topics (hsv//eng)
Keyword
- whole-genome sequencing
- carbapenem resistance
- genotype-phenotype association
- human
- metallo-beta-lactamase
- complete nucleotide-sequence
- pseudomonas-aeruginosa
- antibiotic-resistance
- escherichia-coli
- antimicrobial resistance
- klebsiella-pneumoniae
- topoisomerase-iv
- quinolone-resistance
- bacterial-resistance
- carbapenem resistance
- genotype–phenotype association
- human pathogens
Publication and Content Type
- ref (subject category)
- art (subject category)
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Johnning, Anna, ...
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Karami, Nahid, 1 ...
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Tång Hallbäck, E ...
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Müller, Vilhelm, ...
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Nyberg, Lena, 19 ...
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Buongermino Pere ...
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Stewart, C.
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Ambjörnsson, Tob ...
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Westerlund, Fred ...
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Adlerberth, Inge ...
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Kristiansson, Er ...
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MEDICAL AND HEAL ...
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NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biophysics
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NATURAL SCIENCES
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Microbial Genomi ...
- By the university
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University of Gothenburg
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Chalmers University of Technology
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Lund University