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Bacterial Outer Mem...
Bacterial Outer Membrane Vesicles Induce Vitronectin Release Into the Bronchoalveolar Space Conferring Protection From Complement-Mediated Killing
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- Paulsson, Magnus (author)
- Lund University,Lunds universitet,Klinisk mikrobiologi, Malmö,Forskargrupper vid Lunds universitet,Clinical Microbiology, Malmö,Lund University Research Groups
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- Che, K. F. (author)
- Karolinska Institutet
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- Ahl, Jonas (author)
- Lund University,Lunds universitet,Enheten för infektionssjukdomar,Forskargrupper vid Lunds universitet,Infectious Diseases Research Unit,Lund University Research Groups
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- Tham, Johan (author)
- Lund University,Lunds universitet,Enheten för infektionssjukdomar,Forskargrupper vid Lunds universitet,Infectious Diseases Research Unit,Lund University Research Groups
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- Sandblad, Linda (author)
- Umeå University,Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Smith, Margaretha E. (author)
- University of Gothenburg,Gothenburg University,Göteborgs universitet,Institutionen för medicin,Institute of Medicine
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- Qvarfordt, Ingemar, 1954 (author)
- University of Gothenburg,Gothenburg University,Göteborgs universitet,Institutionen för medicin,Institute of Medicine
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- Su, Yu Ching (author)
- Lund University,Lunds universitet,Klinisk mikrobiologi, Malmö,Forskargrupper vid Lunds universitet,Clinical Microbiology, Malmö,Lund University Research Groups
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- Linden, A. (author)
- Karolinska Institutet,Karolinska University Hospital
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- Riesbeck, Kristian (author)
- Lund University,Lunds universitet,Klinisk mikrobiologi, Malmö,Forskargrupper vid Lunds universitet,Clinical Microbiology, Malmö,Lund University Research Groups
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(creator_code:org_t)
- 2018-07-13
- 2018
- English.
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In: Frontiers in Microbiology. - : Frontiers Media SA. - 1664-302X. ; 9
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Abstract
Subject headings
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- Pathogens causing pneumonia utilize the complement regulator vitronectin to evade complement-mediated killing. Although vitronectin is associated with several chronic lung diseases, the role of bronchoalveolar vitronectin in pneumonia has not been studied. This study sought to reveal the involvement of vitronectin in the bronchoalveolar space during pneumonia, to assess the effect of outer membrane vesicles and endotoxin on vitronectin release, and to determine whether bacterial pathogens utilize pulmonary vitronectin for evasion. Vitronectin was analyzed in cell-free bronchoalveolar lavage fluid harvested from patients with pneumonia (n = 8) and from healthy volunteers after subsegmental endotoxin instillation (n = 13). Vitronectin binding by Pseudomonas aeruginosa and Haemophilus influenzae was analyzed, and subsequent complement evasion was assessed by serum challenge. The effects of outer membrane vesicles on vitronectin production in mouse lungs and human type II alveolar epithelial cells (A549) were determined. We detected increased vitronectin concentrations in lavage fluid during pneumonia (p = 0.0063) and after bronchial endotoxin challenge (p = 0.016). The capture of vitronectin by bacteria significantly reduced complement-mediated lysis. Following challenge with vesicles, vitronectin was detected in mouse bronchoalveolar space, and mouse alveolar epithelial cells in vivo as well as A549 cells in vitro contained increased levels of vitronectin. Taken together, outer membrane vesicles and endotoxin from Gram-negative bacteria induce vitronectin, which is released into the bronchoalveolar space, and used for evasion of complement-mediated clearance.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Lungmedicin och allergi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Respiratory Medicine and Allergy (hsv//eng)
Keyword
- alveolar epithelial cells
- complement regulators
- endotoxin
- outer
- membrane vesicles
- Haemophilus influenzae
- immune evasion
- pneumonia
- Pseudomonas aeruginosa
- nontypable haemophilus-influenzae
- streptococcus-pneumoniae
- pseudomonas-aeruginosa
- serum resistance
- lavage fluid
- disease
- cells
- immunity
- lung
- sarcoidosis
- alveolar epithelial cells
- Alveolar epithelial cells
- Complement regulators
- Endotoxin
- Immune evasion
- Outer membrane vesicles
- Pneumonia
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Paulsson, Magnus
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Che, K. F.
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Ahl, Jonas
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Tham, Johan
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Sandblad, Linda
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Smith, Margareth ...
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show more...
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Qvarfordt, Ingem ...
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Su, Yu Ching
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Linden, A.
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Riesbeck, Kristi ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Microbiology in ...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Respiratory Medi ...
- Articles in the publication
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Frontiers in Mic ...
- By the university
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University of Gothenburg
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Umeå University
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Karolinska Institutet
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Lund University