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Direct Measurement ...
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Ranjbari, EliasGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg
(author)
Direct Measurement of Total Vesicular Catecholamine Content with Electrochemical Microwell Arrays
- Article/chapterEnglish2020
Publisher, publication year, extent ...
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2020-07-21
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American Chemical Society (ACS),2020
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LIBRIS-ID:oai:gup.ub.gu.se/297795
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https://gup.ub.gu.se/publication/297795URI
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https://doi.org/10.1021/acs.analchem.0c02010DOI
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https://research.chalmers.se/publication/519035URI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
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We have designed and fabricated a microwell array chip (MWAC) to trap and detect the entire content of individual vesicles after disruption of the vesicular membrane by an applied electrical potential. To understand the mechanism of vesicle impact electrochemical cytometry (VIEC) in microwells, we simulated the rupture of the vesicles and subsequent diffusion of entrapped analytes. Two possibilities were tested: (i) the vesicle opens toward the electrode, and (ii) the vesicle opens away from the electrode. These two possibilities were simulated in the different microwells with varied depth and width. Experimental VIEC measurements of the number of molecules for each vesicle in the MWAC were compared to VIEC on a gold microdisk electrode as a control, and the quantified catecholamines between these two techniques was the same. We observed a prespike foot in a significant number of events (similar to 20%) and argue this supports the hypothesis that the vesicles rupture toward the electrode surface with a more complex mechanism including the formation of a stable pore intermediate. This study not only confirms that in standard VIEC experiments the whole content of the vesicle is oxidized and quantified at the surface of the microdisk electrode but actively verifies that the adsorbed vesicle on the surface of the electrode forms a pore in the vicinity of the electrode rather than away from it. The fabricated MWAC promotes our ability to quantify the content of vesicles accurately, which is fundamentally important in bioanalysis of the vesicles.
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Taleat, ZahraGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg
(author)
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Mapar, Mokhtar,1983Chalmers tekniska högskola,Chalmers University of Technology(Swepub:cth)mapar
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Aref, Mohaddeseh A.Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg(Swepub:gu)xamimo
(author)
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Dunevall, JohanGothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg(Swepub:cth)dunevall
(author)
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Ewing, Andrew G,1957Gothenburg University,Göteborgs universitet,Institutionen för kemi och molekylärbiologi,Department of Chemistry and Molecular Biology,University of Gothenburg(Swepub:gu)xewian
(author)
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Göteborgs universitetInstitutionen för kemi och molekylärbiologi
(creator_code:org_t)
Related titles
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In:Analytical Chemistry: American Chemical Society (ACS)92:16, s. 11325-113310003-27001520-6882
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