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A method for 2D rec...
A method for 2D reconstruction of intracellularly labeled neurons from sequential sections.
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- Skoglund, Thomas, 1969 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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- Hammar, Ingela, 1964 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi,Institute of Physiology and Pharmacology
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- Olsson, C (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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visa fler...
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- Berthold, Claes-Henric, 1937 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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visa färre...
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(creator_code:org_t)
- 1994
- 1994
- Engelska.
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Ingår i: Journal of neuroscience methods. - 0165-0270. ; 53:2, s. 199-202
- Relaterad länk:
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https://gup.ub.gu.se...
Abstract
Ämnesord
Stäng
- A technique for 2D reconstruction of intracellularly labeled neurons from sequential sections is described. The system consists of a Charged Coupled Device-camera mounted on a microscope, a videomixer and a IBM-compatible PC with a framegrabber. The neurons (interneurons from the spinal cord of the cat) were labeled iontophoretically by horshradish peroxidase and subsequently cut in 60 microns sections. The sections were aligned using the video mixer by fitting the cut dendrites and axon from one section with their counterparts in the following section. The images were then digitized in the PC where they were fused to create a superimposed picture of the aligned parts of the neuron; a 2D reconstruction was created.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Fysiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Physiology (hsv//eng)
Nyckelord
- Animals
- Axons
- ultrastructure
- Cats
- Dendrites
- ultrastructure
- Horseradish Peroxidase
- Image Processing
- Computer-Assisted
- instrumentation
- methods
- Interneurons
- ultrastructure
- Microscopy
- Video
- Neurons
- ultrastructure
- Software
- Spinal Cord
- cytology
- ultrastructure
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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