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Cholera toxin induc...
Cholera toxin induces a transient depletion of CD8+ intraepithelial lymphocytes in the rat small intestine as detected by microarray and immunohistochemistry.
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- Flach, Carl-Fredrik, 1977 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk mikrobiologi och immunologi,Institute of Medical Microbiology/Immunology
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- Lange, Stefan, 1948 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för laboratoriemedicin, Avdelningen för klinisk bakteriologi,Institute of Laboratory Medicine, Dept of Clinical Bacteriology
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- Jennische, Eva, 1949 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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- Lönnroth, Ivar, 1940 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk mikrobiologi och immunologi,Institutionen för laboratoriemedicin, Avdelningen för klinisk bakteriologi,Institute of Medical Microbiology/Immunology,Institute of Laboratory Medicine, Dept of Clinical Bacteriology
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- Holmgren, Jan, 1944 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för medicinsk mikrobiologi och immunologi,Institute of Medical Microbiology/Immunology
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(creator_code:org_t)
- 2005
- 2005
- Engelska.
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Ingår i: Infection and immunity. - 0019-9567. ; 73:9, s. 5595-602
- Relaterad länk:
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Cholera toxin (CT), besides causing intestinal hypersecretion after intragastric administration or during cholera infection, affects a multitude of regulatory mechanisms within the gut mucosal network, including T cells. By use of microarray screening, real-time PCR, and immunohistochemistry, we demonstrate here a rapid depletion of jejunal CD8(+) intraepithelial lymphocytes (IEL) in rats after intragastric CT challenge. This depletion may depend on CT-induced migration of IEL, since it was associated with a progressive decrease of CD8(+) cells in the epithelium and a contemporary transient increase of such cells, preferentially at the base of the villi, in the lamina propria. A significant decrease in the total number of villous CD8(+) cells at 6 and 18 h after CT challenge was detected; this possibly reflects an efflux from the jejunal mucosa. The kinetics of the CD8(+) IEL demonstrate the return to normal intraepithelial position at original numbers already 72 h after the single CT dose. The induced migration seems to be dependent on the enzymatic A-subunit of CT, since challenge with neither sorbitol nor CT B-subunit did mimic the effects of CT on CD8(+) IEL. Furthermore, a decrease in the level of both RANTES transcript and protein was detected, most likely as a consequence of the CT-induced migration of CD8(+) IEL. These results point to a complex interaction between CT, epithelial cells, and IEL, resulting in a disturbance of the gut homeostasis, which might have relevance for the strong immunomodulatory effects of intragastrically administered CT.
Nyckelord
- Animals
- CD8-Positive T-Lymphocytes
- immunology
- Cholera Toxin
- pharmacology
- Immunohistochemistry
- Intestinal Mucosa
- chemistry
- cytology
- immunology
- Jejunum
- chemistry
- cytology
- immunology
- Lymphocyte Depletion
- Male
- Oligonucleotide Array Sequence Analysis
- RANTES
- biosynthesis
- genetics
- RNA
- Messenger
- biosynthesis
- Rats
- Rats
- Sprague-Dawley
- Reverse Transcriptase Polymerase Chain Reaction
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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