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Adenovirus 5 vector...
Adenovirus 5 vector genetically re-targeted by an Affibody molecule with specificity for tumor antigen HER2/neu.
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- Magnusson, Maria K, 1972 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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- Henning, Petra, 1974 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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- Myhre, Susanna, 1974 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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Wikman, M (author)
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Uil, T G (author)
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Friedman, M (author)
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- Ståhl, Stefan (author)
- KTH,Molekylär Bioteknologi
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Hoeben, R C (author)
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Habib, N A (author)
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Ståhl, S (author)
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Boulanger, P (author)
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- Lindholm, Leif, 1940 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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Andersson, Karin, 1972 (author)
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(creator_code:org_t)
- 2007-02-02
- 2007
- English.
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In: Cancer gene therapy. - : Springer Science and Business Media LLC. - 0929-1903 .- 1476-5500. ; 14:5, s. 468-79
- Related links:
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https://www.nature.c...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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https://urn.kb.se/re...
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Abstract
Subject headings
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- In order to use adenovirus (Ad) type 5 (Ad5) for cancer gene therapy, Ad needs to be de-targeted from its native receptors and re-targeted to a tumor antigen. A limiting factor for this has been to find a ligand that (i) binds a relevant target, (ii) is able to fold correctly in the reducing environment of the cytoplasm and (iii) when incorporated at an optimal position on the virion results in a virus with a low physical particle to plaque-forming units ratio to diminish the viral load to be administered to a future patient. Here, we present a solution to these problems by producing a genetically re-targeted Ad with a tandem repeat of the HER2/neu reactive Affibody molecule (ZH) in the HI-loop of a Coxsackie B virus and Ad receptor (CAR) binding ablated fiber genetically modified to contain sequences for flexible linkers between the ZH and the knob sequences. ZH is an Affibody molecule specific for the extracellular domain of human epidermal growth factor receptor 2 (HER2/neu) that is overexpressed in inter alia breast and ovarian carcinomas. The virus presented here exhibits near wild-type growth characteristics, infects cells via HER2/neu instead of CAR and represents an important step toward the development of genetically re-targeted adenoviruses with clinical relevance.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- Adenoviridae
- genetics
- Antigens
- Neoplasm
- genetics
- immunology
- Breast Neoplasms
- therapy
- Female
- Gene Therapy
- methods
- Genetic Vectors
- genetics
- Humans
- Ligands
- Ovarian Neoplasms
- therapy
- Receptor
- erbB-2
- genetics
- immunology
- Recombinant Fusion Proteins
- genetics
- immunology
- Tumor Cells
- Cultured
- adenovirus
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Magnusson, Maria ...
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Henning, Petra, ...
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Myhre, Susanna, ...
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Wikman, M
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Uil, T G
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Friedman, M
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show more...
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Ståhl, Stefan
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Hoeben, R C
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Habib, N A
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Ståhl, S
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Boulanger, P
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Lindholm, Leif, ...
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Andersson, Karin ...
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Microbiology in ...
- Articles in the publication
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Cancer gene ther ...
- By the university
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University of Gothenburg
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Royal Institute of Technology