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Body fat content ca...
Body fat content can be predicted in vivo in mice using a modified dual-energy X-ray absorptiometry technique.
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- Sjögren, Klara, 1970 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine
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Hellberg, N (author)
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- Bohlooly-Yeganeh, Mohammad, 1966 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för fysiologi och farmakologi,Institute of Physiology and Pharmacology
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- Savendahl, L. (author)
- Karolinska Institutet
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Johansson, M S (author)
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Berglindh, T (author)
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- Bosaeus, Ingvar, 1950 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för klinisk näringslära,Institute of Internal Medicine, Dept of Clinical Nutrition
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- Ohlsson, Claes, 1965 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för internmedicin,Institute of Internal Medicine, Dept of Medicine
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(creator_code:org_t)
- Elsevier BV, 2001
- 2001
- English.
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In: The Journal of nutrition. - : Elsevier BV. - 0022-3166. ; 131:11, s. 2963-6
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Abstract
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- The introduction of transgenic mice as animal models in medical research has increased the need for methods to study the phenotype of mice. The aim of the present study was to develop and evaluate a method for in vivo prediction of fat content in living mice. We combined a modified dual-energy X-ray technique with an image analysis procedure. This combined procedure calculates the percentage of fat area, defined as the percentage of the total area of the mice consisting of >50% fat. A high correlation between the percentage of fat area and dissected adipose tissue was seen in both male and female mice (males, r = 0.92, P < 0.001; females, r = 0.88, P < 0.001). A high correlation was also seen between the percentage of fat area and serum levels of leptin (males, r = 0.95, P < 0.001; females, r = 0.86, P < 0.001). An additional experiment demonstrated a very strong correlation between the percentage of fat area and total body fat as determined by chemical extraction (r = 0.97, P < 0.001). In summary, the percentage of fat area, as measured with the dual-energy X-ray/image combined procedure, provides a good in vivo estimation of total body fat content in mice.
Keyword
- Absorptiometry
- Photon
- Adipose Tissue
- Animals
- Body Mass Index
- Enzyme-Linked Immunosorbent Assay
- Female
- Leptin
- blood
- Linear Models
- Male
- Mice
- Mice
- Inbred C57BL
- Predictive Value of Tests
Publication and Content Type
- ref (subject category)
- art (subject category)
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Sjögren, Klara, ...
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Hellberg, N
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Bohlooly-Yeganeh ...
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Savendahl, L.
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Johansson, M S
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Berglindh, T
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show more...
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Bosaeus, Ingvar, ...
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Ohlsson, Claes, ...
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The Journal of n ...
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University of Gothenburg
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Karolinska Institutet