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Abnormal reactivity...
Abnormal reactivity of muller cells after retinal detachment in mice deficient in GFAP and vimentin.
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Verardo, Mark R (författare)
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Lewis, Geoffrey P (författare)
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Takeda, Masumi (författare)
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Linberg, Kenneth A (författare)
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Byun, Jiyun (författare)
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Luna, Gabriel (författare)
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- Wilhelmsson, Ulrika, 1970 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för klinisk neurovetenskap och rehabilitering,Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation
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- Pekny, Milos, 1965 (författare)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för klinisk neurovetenskap och rehabilitering,Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation
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Chen, Dong-Feng (författare)
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Fisher, Steven K (författare)
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(creator_code:org_t)
- Association for Research in Vision and Ophthalmology (ARVO), 2008
- 2008
- Engelska.
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Ingår i: Investigative ophthalmology & visual science. - : Association for Research in Vision and Ophthalmology (ARVO). - 1552-5783. ; 49:8, s. 3659-65
- Relaterad länk:
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https://europepmc.or...
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https://gup.ub.gu.se...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- PURPOSE: To determine the roles of glial fibrillary acidic protein (GFAP) and vimentin in M?ller cell reactivity. METHODS: Retinal detachments were created in mice deficient for GFAP and vimentin (GFAP(-/-)vim(-/-)) and age-matched wild-type (wt) mice. The reactivity of the retina was studied by immunofluorescence and electron microscopy. RESULTS: M?ller cell morphology was different and glutamine synthetase immunoreactivity was reduced in the undisturbed GFAP(-/-)vim(-/-) retinas. After retinal detachment, M?ller cells formed subretinal glial scars in the wt mice. In contrast, such scars were not observed in GFAP(-/-)vim(-/-) mice. M?ller cells, which normally elongate and thicken in response to detachment, appeared compressed, thin, and "spikey" in the GFAP(-/-)vim(-/-) mice. The end foot region of M?ller cells in the GFAP(-/-)vim(-/-) mice often sheared away from the rest of the retina during detachment, corroborating earlier results showing decreased resistance of this region in GFAP(-/-)vim(-/-) retinas to mechanical stress. In regions with end foot shearing, ganglion cells showed intense neurite sprouting, as revealed by anti-neurofilament labeling, a response rarely observed in wt mice. CONCLUSIONS: M?ller cells are subtly different in the GFAP(-/-)vim(-/-) mouse retina before detachment. The end foot region of these cells may be structurally reinforced by the presence of the intermediate filament cytoskeleton, and our data suggest a critical role for these proteins in M?ller cell reaction to retinal detachment and participation in subretinal gliosis.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Neurovetenskaper (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Neurosciences (hsv//eng)
Nyckelord
- Animals
- Disease Models
- Animal
- Fluorescent Antibody Technique
- Indirect
- Glutamate-Ammonia Ligase
- metabolism
- Male
- Mice
- Mice
- Inbred C57BL
- Mice
- Knockout
- Microscopy
- Confocal
- Microscopy
- Electron
- Transmission
- Nerve Tissue Proteins
- physiology
- Neuroglia
- metabolism
- pathology
- Photoreceptor Cells
- Vertebrate
- metabolism
- pathology
- Retina
- metabolism
- pathology
- Retinal Bipolar Cells
- metabolism
- pathology
- Retinal Detachment
- metabolism
- pathology
- Retinal Ganglion Cells
- metabolism
- pathology
- Retinal Horizontal Cells
- metabolism
- pathology
- Rod Opsins
- metabolism
- S100 Proteins
- metabolism
- Up-Regulation
- Vimentin
- physiology
Publikations- och innehållstyp
- ref (ämneskategori)
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Verardo, Mark R
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Lewis, Geoffrey ...
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Takeda, Masumi
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Linberg, Kenneth ...
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Byun, Jiyun
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Luna, Gabriel
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visa fler...
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Wilhelmsson, Ulr ...
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Pekny, Milos, 19 ...
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Chen, Dong-Feng
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Fisher, Steven K
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Neurovetenskaper
- Artiklar i publikationen
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Investigative op ...
- Av lärosätet
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Göteborgs universitet