Sökning: onr:"swepub:oai:lup.lub.lu.se:0e80b0b2-af49-4a08-b826-ca77bbd8819a" > Rapid microwave-sti...
Fältnamn | Indikatorer | Metadata |
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000 | 03489naa a2200433 4500 | |
001 | oai:lup.lub.lu.se:0e80b0b2-af49-4a08-b826-ca77bbd8819a | |
003 | SwePub | |
008 | 230210s1997 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/0e80b0b2-af49-4a08-b826-ca77bbd8819a2 URI |
024 | 7 | a https://doi.org/10.1002/(SICI)1096-9896(199711)183:3<369::AID-PATH929>3.0.CO;2-92 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Ruijter, Emiel Thu Radboud University Medical Center4 aut |
245 | 1 0 | a Rapid microwave-stimulated fixation of entire prostatectomy specimens |
264 | 1 | c 1997 |
520 | a Conventional fixation of large solid surgical specimens is a slow process. Consequently, autolytic damage to tissues may occur if the fixative does not reach the central part of the specimen in time. However, as there is also a time relationship between formalin fixation and antigen masking, fixation for too long can also be detrimental. In seeking the optimum balance for fixation, microwave irradiation might be of assistance. This study set out to evaluate methods for fixing entire prostate glands within a brief period of time, using microwave-stimulated formalin fixation. The results show that entire prostates can be optimally fixed if formalin is present throughout the tissue as the temperature is increased by microwave irradiation. This is achieved by injecting the fixative into the prostate at multiple sites immediately following prostatectomy. The technique described ensures standardization of a critical step during tissue processing, leading to uniform microscopic results with both routine and immunohistochemical stains. It is a simple, rapid method, suitable for routine diagnostic use. Using this modified approach, DNA of much larger sizes can be extracted from paraffin-embedded material, which could expand the possibilities for molecular analysis. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Klinisk medicinx Cancer och onkologi0 (SwePub)302032 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Clinical Medicinex Cancer and Oncology0 (SwePub)302032 hsv//eng |
653 | a Fixation | |
653 | a Immunohistochemistry | |
653 | a Microwaves | |
653 | a Prostate | |
700 | 1 | a Miller, Gary J.u University of Colorado4 aut |
700 | 1 | a Aalders, Tilly W.u Radboud University Medical Center4 aut |
700 | 1 | a Van De Kaa, Christina A.u Radboud University Medical Center4 aut |
700 | 1 | a Schalken, Jack A.u Radboud University Medical Center4 aut |
700 | 1 | a Debruyne, Frans M.u Radboud University Medical Center4 aut |
700 | 1 | a Boon, Mathilde E.u Leiden Cytology and Pathology Laboratory4 aut |
700 | 1 | a Schröder, F. H.4 cre |
700 | 1 | a Lilja, H.u Lund University,Lunds universitet,Klinisk kemi, Malmö,Forskargrupper vid Lunds universitet,Clinical Chemistry, Malmö,Lund University Research Groups4 cre0 (Swepub:lu)klke-hli |
700 | 1 | a Robson, C. N.4 cre |
710 | 2 | a Radboud University Medical Centerb University of Colorado4 org |
773 | 0 | t Journal of Pathologyg 183:3, s. 369-375q 183:3<369-375x 0022-3417 |
856 | 4 | u http://dx.doi.org/10.1002/(SICI)1096-9896(199711)183:3<369::AID-PATH929>3.0.CO;2-9y FULLTEXT |
856 | 4 8 | u https://lup.lub.lu.se/record/0e80b0b2-af49-4a08-b826-ca77bbd8819a |
856 | 4 8 | u https://doi.org/10.1002/(SICI)1096-9896(199711)183:3<369::AID-PATH929>3.0.CO;2-9 |
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