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Imaging meets cytom...
Imaging meets cytometry : Analyzing heterogeneous functional microscopic data from living cell populations
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- Draper, Matthew (författare)
- University of Ulster
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- Willems, Mara (författare)
- University of Oxford,Churchill Hospital
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- Malahe, Reshwan K. (författare)
- Churchill Hospital,University of Oxford
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- Hamilton, Alexander (författare)
- Lund University,Lunds universitet,Diabetes - molekylär metabolism,Forskargrupper vid Lunds universitet,Diabetes - Molecular Metabolism,Lund University Research Groups,Churchill Hospital,University of Oxford,Skåne University Hospital
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- Tarasov, Andrei I. (författare)
- University of Oxford,University of Ulster,Churchill Hospital
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(creator_code:org_t)
- 2021-01-13
- 2021
- Engelska.
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Ingår i: Journal of Imaging. - : MDPI AG. - 2313-433X. ; 7:1
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://www.mdpi.com...
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https://lup.lub.lu.s...
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https://doi.org/10.3...
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Abstract
Ämnesord
Stäng
- Biological tissue consists of populations of cells exhibiting different responses to pharmacological stimuli. To probe the heterogeneity of cell function, we propose a multiplexed approach based on real-time imaging of the secondary messenger levels within each cell of the tissue, followed by extraction of the changes of single-cell fluorescence over time. By utilizing a piecewise baseline correction, we were able to quantify the effects of multiple pharmacological stimuli added and removed sequentially to pancreatic islets of Langerhans, thereby performing a deep functional profiling for each cell within the islet. Cluster analysis based on the functional profile demonstrated dose-dependent changes in statistical inter-relationships between islet cell populations. We therefore believe that the functional cytometric approach can be used for routine quantitative profiling of the tissue for drug screening or pathological testing.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Radiologi och bildbehandling (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Radiology, Nuclear Medicine and Medical Imaging (hsv//eng)
Nyckelord
- Antidiabetic drugs
- Baseline correction
- Cell profiling
- Fluorescence
- Time-lapse imaging
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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