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Effects of Ice Nucl...
Effects of Ice Nucleation Protein Repeat Number and Oligomerization Level on Ice Nucleation Activity
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- Ling, M. L. (författare)
- Aarhus University
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- Wex, H. (författare)
- Leibniz Institute for Tropospheric Research (TROPOS)
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- Grawe, S. (författare)
- Leibniz Institute for Tropospheric Research (TROPOS)
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- Jakobsson, J. (författare)
- Lund University,Lunds universitet,Ergonomi och aerosolteknologi,Institutionen för designvetenskaper,Institutioner vid LTH,Lunds Tekniska Högskola,Ergonomics and Aerosol Technology,Department of Design Sciences,Departments at LTH,Faculty of Engineering, LTH
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- Löndahl, J. (författare)
- Lund University,Lunds universitet,Ergonomi och aerosolteknologi,Institutionen för designvetenskaper,Institutioner vid LTH,Lunds Tekniska Högskola,MERGE: ModElling the Regional and Global Earth system,Centrum för miljö- och klimatvetenskap (CEC),Naturvetenskapliga fakulteten,Ergonomics and Aerosol Technology,Department of Design Sciences,Departments at LTH,Faculty of Engineering, LTH,Centre for Environmental and Climate Science (CEC),Faculty of Science
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- Hartmann, S. (författare)
- Leibniz Institute for Tropospheric Research (TROPOS)
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- Finster, K. (författare)
- Aarhus University
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- Boesen, T. (författare)
- Aarhus University
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- Šantl-Temkiv, T. (författare)
- Lund University,Lunds universitet,Ergonomi och aerosolteknologi,Institutionen för designvetenskaper,Institutioner vid LTH,Lunds Tekniska Högskola,Ergonomics and Aerosol Technology,Department of Design Sciences,Departments at LTH,Faculty of Engineering, LTH,Aarhus University
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(creator_code:org_t)
- 2018
- 2018
- Engelska.
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Ingår i: Journal of Geophysical Research: Atmospheres. - 2169-8996. ; 123:3, s. 1802-1810
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Ice nucleation active bacteria have attracted particular attention due to their unique ability to produce specific ice nucleation proteins (INpros), which are the most efficient ice nuclei known as they induce nucleation at temperatures close to 0°C. Our model bacterium Pseudomonas syringae strain R10.79 produced INpros containing 67 tandem repeats, forming the proposed ice-binding surface. To understand the role of the INpro repeats as well as the role of intermolecular interactions between INpros for their ice nucleation behavior, we produced a truncated version of the protein with only 16 tandem repeats (INpro16R). The purified INpro16R produced oligomers of varying sizes. Immersion freezing ice nucleation behavior of purified INpro16R was characterized by droplet-freezing assays and in the Leipzig Aerosol Cloud Interaction Simulator. Predominant INpro16R oligomers introduced into Leipzig Aerosol Cloud Interaction Simulator as single particles with diameters of 50 nm or 70 nm were ice nucleation active at temperatures of -26°C and -24°C, respectively. These are much lower temperatures compared to that of intact INpros (-12°C). The data clearly indicated that the number of repeats determines the ice nucleation temperature. In addition, ice nucleation between -9°C and -10°C, comparable to the activity of intact INpro, was caused by higher-order INpro16R oligomers. This supported previous observations that INpro oligomerization increases the ice-binding surface, thereby affecting ice nucleation activity. In conclusion, both repeat number and oligomerization contribute in a seemingly independent manner to the nucleation mechanism of INpros.
Ämnesord
- NATURVETENSKAP -- Geovetenskap och miljövetenskap -- Meteorologi och atmosfärforskning (hsv//swe)
- NATURAL SCIENCES -- Earth and Related Environmental Sciences -- Meteorology and Atmospheric Sciences (hsv//eng)
Nyckelord
- Bioaerosols
- Precipitation
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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