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Globular structures...
Globular structures in roots accumulate phosphorus to extremely high concentrations following phosphorus addition
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- Ryan, Megan H. (author)
- University of Western Australia, Crawley
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- Kaur, Parwinder (author)
- University of Western Australia, Crawley
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- Nazeri, Nazanin K. (author)
- University of Western Australia, Crawley
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- Clode, Peta L. (author)
- University of Western Australia, Crawley
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Keeble-Gagnère, Gabriel (author)
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- Doolette, Ashlea L. (author)
- University of Adelaide
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- Smernik, Ronald J. (author)
- University of Adelaide
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- Van Aken, Olivier (author)
- Lund University,Lunds universitet,Molekylär cellbiologi,Biologiska institutionen,Naturvetenskapliga fakulteten,Molecular Cell Biology,Department of Biology,Faculty of Science,University of Western Australia, Crawley
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- Nicol, Dion (author)
- University of Western Australia, Crawley
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- Maruyama, Hayato (author)
- Hokkaido University
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- Ezawa, Tatsuhiro (author)
- Hokkaido University
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- Lambers, Hans (author)
- University of Western Australia, Crawley
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- Millar, A. Harvey (author)
- University of Western Australia, Crawley
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- Appels, Rudi (author)
- University of Melbourne
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(creator_code:org_t)
- 2019-03-26
- 2019
- English.
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In: Plant, Cell and Environment. - : Wiley. - 0140-7791 .- 1365-3040. ; 42:6, s. 1987-2002
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Abstract
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- Crops with improved uptake of fertilizer phosphorus (P) would reduce P losses and confer environmental benefits. We examined how P-sufficient 6-week-old soil-grown Trifolium subterraneum plants, and 2-week-old seedlings in solution culture, accumulated P in roots after inorganic P (Pi) addition. In contrast to our expectation that vacuoles would accumulate excess P, after 7 days, X-ray microanalysis showed that vacuolar [P] remained low (<12 mmol kg −1 ). However, in the plants after P addition, some cortex cells contained globular structures extraordinarily rich in P (often >3,000 mmol kg −1 ), potassium, magnesium, and sodium. Similar structures were evident in seedlings, both before and after P addition, with their [P] increasing threefold after P addition. Nuclear magnetic resonance (NMR) spectroscopy showed seedling roots accumulated Pi following P addition, and transmission electron microscopy (TEM) revealed large plastids. For seedlings, we demonstrated that roots differentially expressed genes after P addition using RNAseq mapped to the T. subterraneum reference genome assembly and transcriptome profiles. Among the most up-regulated genes after 4 hr was TSub_g9430.t1, which is similar to plastid envelope Pi transporters (PHT4;1, PHT4;4): expression of vacuolar Pi-transporter homologs did not change. We suggest that subcellular P accumulation in globular structures, which may include plastids, aids cytosolic Pi homeostasis under high-P availability.
Subject headings
- LANTBRUKSVETENSKAPER -- Bioteknologi med applikationer på växter och djur -- Växtbioteknologi (hsv//swe)
- AGRICULTURAL SCIENCES -- Agricultural Biotechnology -- Plant Biotechnology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Botanik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Botany (hsv//eng)
Keyword
- nuclear magnetic resonance spectroscopy
- phosphorous efficient crops
- plastids
- roots
- vacuoles
- X-ray microanalysis
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Ryan, Megan H.
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Kaur, Parwinder
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Nazeri, Nazanin ...
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Clode, Peta L.
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Keeble-Gagnère, ...
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Doolette, Ashlea ...
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show more...
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Smernik, Ronald ...
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Van Aken, Olivie ...
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Nicol, Dion
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Maruyama, Hayato
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Ezawa, Tatsuhiro
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Lambers, Hans
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Millar, A. Harve ...
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Appels, Rudi
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show less...
- About the subject
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- AGRICULTURAL SCIENCES
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AGRICULTURAL SCI ...
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and Agricultural Bio ...
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and Plant Biotechnol ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Botany
- Articles in the publication
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Plant, Cell and ...
- By the university
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Lund University