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Evaluation of 92 ca...
Evaluation of 92 cardiovascular proteins in dried blood spots collected under field-conditions : Off-the-shelf affinity-based multiplexed assays work well, allowing for simplified sample collection
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- Broberg, Karin (författare)
- Karolinska Institutet,Karolinska Institute
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- Grahn, Karin (författare)
- Karolinska Institutet,Karolinska Institute
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- Assarsson, Eva (författare)
- Lund University,Lunds universitet,Avdelningen för arbets- och miljömedicin,Institutionen för laboratoriemedicin,Medicinska fakulteten,Genetisk arbets- och miljömedicin,Forskargrupper vid Lunds universitet,Division of Occupational and Environmental Medicine, Lund University,Department of Laboratory Medicine,Faculty of Medicine,Genetic Occupational and Environmental Medicine,Lund University Research Groups
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- Åberg, Mikael (författare)
- Uppsala University
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- Selander, Jenny (författare)
- Karolinska Institutet,Karolinska Institute
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- Enroth, Stefan, 1976- (författare)
- Uppsala universitet,Uppsala University,Science for Life Laboratory, SciLifeLab,Medicinsk genetik och genomik,Institutionen för cell- och molekylärbiologi,Gyllensten
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- Svensson, Johanna (författare)
- Uppsala universitet,Uppsala University,Science for Life Laboratory (SciLifeLab),Uppsala kliniska forskningscentrum (UCR)
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(creator_code:org_t)
- 2021-02-15
- 2021
- Engelska.
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Ingår i: BioEssays. - : Wiley. - 0265-9247 .- 1521-1878. ; 43:9
- Relaterad länk:
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http://dx.doi.org/10... (free)
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Workplace-collected blood spots deposited on filter paper were analysed with multiplexed affinity-based protein assays and found to be suitable for proteomics analysis. The protein extension assay (PEA) was used to characterize 92 proteins using 1.2 mm punches in repeated samples collected from 20 workers. Overall, 97.8% of the samples and 91.3% of the analysed proteins passed quality control. Both within and between spot correlations using six replicates from the same individual were above 0.99, suggesting that comparable levels are obtained from multiple punches from the same spot and from consecutive spots. Protein levels from dried blood and wet serum from the same individuals were compared and the majority of the analysed proteins were found to be significantly correlated. These results open up for simplified sample collection of blood in field conditions for proteomic analysis, but also highlight that not all proteins can be robustly measured from dried whole blood.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinsk bioteknologi -- Medicinsk bioteknologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Medical Biotechnology -- Medical Biotechnology (hsv//eng)
Nyckelord
- affinity based proteomics
- dried blood spots
- sample collection
- affinity based proteomics
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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