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Pigs, unlike mice, ...
Pigs, unlike mice, have two distinct colonic stem cell populations similar to humans that respond to high-calorie diet prior to insulin resistance
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- Charepalli, Venkata (författare)
- Pennsylvania State University
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- Reddivari, Lavanya (författare)
- Pennsylvania State University
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- Radhakrishnan, Sridhar (författare)
- Pennsylvania State University
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- Eriksson, Elisabeth (författare)
- Lund University,Lunds universitet,Avdelningen för livsmedel och läkemedel,Institutionen för processteknik och tillämpad biovetenskap,Institutioner vid LTH,Lunds Tekniska Högskola,Division of Food and Pharma,Department of Process and Life Science Engineering,Departments at LTH,Faculty of Engineering, LTH
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- Xiao, Xia (författare)
- Pennsylvania State University
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- Kim, Sung Woo (författare)
- North Carolina State University
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- Shen, Frank (författare)
- Pennsylvania State University
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- Vijay-Kumar, Matam (författare)
- Pennsylvania State University
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- Li, Qunhua (författare)
- Pennsylvania State University
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- Bhat, Vadiraja B. (författare)
- Agilent Technologies, US
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- Knight, Rob (författare)
- University of California, San Diego
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- Vanamala, Jairam K P (författare)
- Pennsylvania State University
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(creator_code:org_t)
- 2017
- 2017
- Engelska 9 s.
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Ingår i: Cancer Prevention Research. - 1940-6207. ; 10:8, s. 442-450
- Relaterad länk:
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http://dx.doi.org/10...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Basal colonic crypt stem cells are long lived and play a role in colon homeostasis. Previous evidence has shown that high-calorie diet (HCD) enhances colonic stem cell numbers and expansion of the proliferative zone, an important biomarker for colon cancer. However, it is not clear how HCD drives dysregulation of colon stem cell/colonocyte proliferative kinetics. We used a human-relevant pig model and developed an immunofluorescence technique to detect and quantify colonic stem cells. Pigs (n = 8/group) were provided either standard diet (SD; 5% fat) or HCD (23% fat) for 13 weeks. HCD- and SD-consuming pigs had similar total calorie intake, serum iron, insulin, and glucose levels. However, HCD elevated both colonic proliferative zone (KI-67) and stem cell zone (ASCL-2 and BMI-1). Proliferative zone correlated with elevated innate colonic inflammatory markers TLR-4, NF-κB, IL6, and lipocalin-2 (r ≥ 0.62, P = 0.02). Elevated gut bacterial phyla proteobacteria and firmicutes in HCD-consuming pigs correlated with proliferative and stem cell zone. Colonic proteome data revealed the upregulation of proteins involved in cell migration and proliferation and correlated with proliferative and stem cell zone expansion. Our study suggests that pig colon, unlike mice, has two distinct stem cells (ASCL-2 and BMI-1) similar to humans, and HCD increases expansion of colonic proliferative and stem cell zone. Thus, pig model can aid in the development of preventive strategies against gut bacterial dysbiosis and inflammation-promoted diseases, such as colon cancer.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Publikations- och innehållstyp
- art (ämneskategori)
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Till lärosätets databas
- Av författaren/redakt...
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Charepalli, Venk ...
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Reddivari, Lavan ...
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Radhakrishnan, S ...
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Eriksson, Elisab ...
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Xiao, Xia
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Kim, Sung Woo
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visa fler...
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Shen, Frank
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Vijay-Kumar, Mat ...
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Li, Qunhua
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Bhat, Vadiraja B ...
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Knight, Rob
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Vanamala, Jairam ...
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visa färre...
- Om ämnet
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Medicinska och f ...
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och Cell och molekyl ...
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- MEDICIN OCH HÄLSOVETENSKAP
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MEDICIN OCH HÄLS ...
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och Klinisk medicin
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och Cancer och onkol ...
- Artiklar i publikationen
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Cancer Preventio ...
- Av lärosätet
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Lunds universitet