Sökning: onr:"swepub:oai:lup.lub.lu.se:6b8034c4-6268-447d-bd2a-f6e5e313ae29" > Orthotopic Bone For...
Fältnamn | Indikatorer | Metadata |
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000 | 05000naa a2200613 4500 | |
001 | oai:lup.lub.lu.se:6b8034c4-6268-447d-bd2a-f6e5e313ae29 | |
003 | SwePub | |
008 | 220210s2020 | |||||||||||000 ||eng| | |
024 | 7 | a https://lup.lub.lu.se/record/6b8034c4-6268-447d-bd2a-f6e5e313ae292 URI |
024 | 7 | a https://doi.org/10.3390/ijms211972332 DOI |
040 | a (SwePub)lu | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a art2 swepub-publicationtype |
072 | 7 | a ref2 swepub-contenttype |
100 | 1 | a Pigeot, Sébastienu University Hospital Basel4 aut |
245 | 1 0 | a Orthotopic Bone Formation by Streamlined Engineering and Devitalization of Human Hypertrophic Cartilage |
264 | c 2020-09-30 | |
264 | 1 | b MDPI AG,c 2020 |
520 | a Most bones of the human body form and heal through endochondral ossification, whereby hypertrophic cartilage (HyC) is formed and subsequently remodeled into bone. We previously demonstrated that HyC can be engineered from human mesenchymal stromal cells (hMSC), and subsequently devitalized by apoptosis induction. The resulting extracellular matrix (ECM) tissue retained osteoinductive properties, leading to ectopic bone formation. In this study, we aimed at engineering and devitalizing upscaled quantities of HyC ECM within a perfusion bioreactor, followed by in vivo assessment in an orthotopic bone repair model. We hypothesized that the devitalized HyC ECM would outperform a clinical product currently used for bone reconstructive surgery. Human MSC were genetically engineered with a gene cassette enabling apoptosis induction upon addition of an adjuvant. Engineered hMSC were seeded, differentiated, and devitalized within a perfusion bioreactor. The resulting HyC ECM was subsequently implanted in a 10-mm rabbit calvarial defect model, with processed human bone (Maxgraft®) as control. Human MSC cultured in the perfusion bioreactor generated a homogenous HyC ECM and were efficiently induced towards apoptosis. Following six weeks of in vivo implantation, microcomputed tomography and histological analyses of the defects revealed an increased bone formation in the defects filled with HyC ECM as compared to Maxgraft®. This work demonstrates the suitability of engineered devitalized HyC ECM as a bone substitute material, with a performance superior to a state-of-the-art commercial graft. Streamlined generation of the devitalized tissue transplant within a perfusion bioreactor is relevant towards standardized and automated manufacturing of a clinical product. | |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinska och farmaceutiska grundvetenskaperx Cell- och molekylärbiologi0 (SwePub)301082 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Basic Medicinex Cell and Molecular Biology0 (SwePub)301082 hsv//eng |
650 | 7 | a MEDICIN OCH HÄLSOVETENSKAPx Medicinsk bioteknologix Biomaterialvetenskap0 (SwePub)304032 hsv//swe |
650 | 7 | a MEDICAL AND HEALTH SCIENCESx Medical Biotechnologyx Biomaterials Science0 (SwePub)304032 hsv//eng |
653 | a Animals | |
653 | a Apoptosis/genetics | |
653 | a Bone Remodeling/genetics | |
653 | a Bone Substitutes/therapeutic use | |
653 | a Cartilage/growth & development | |
653 | a Cell Differentiation/genetics | |
653 | a Extracellular Matrix/genetics | |
653 | a Humans | |
653 | a Mesenchymal Stem Cell Transplantation | |
653 | a Mesenchymal Stem Cells/cytology | |
653 | a Osteogenesis/genetics | |
653 | a Rabbits | |
653 | a Skull/growth & development | |
653 | a Tissue Engineering/methods | |
653 | a Tissue Scaffolds | |
653 | a Wound Healing/genetics | |
700 | 1 | a Bourgine, Paul Emileu Lund University,Lunds universitet,Molekylär skelettbiologi,Forskargrupper vid Lunds universitet,WCMM- Wallenberg center för molekylär medicinsk forskning,Medicinska fakulteten,Molecular Skeletal Biology,Lund University Research Groups,WCMM-Wallenberg Centre for Molecular Medicine,Faculty of Medicine,University Hospital Basel4 aut0 (Swepub:lu)pa2431bo |
700 | 1 | a Claude, Jaquieryu University Hospital Basel4 aut |
700 | 1 | a Scotti, Celesteu Novartis Institutes for BioMedical Research, Inc.,Istituto Ortopedico Galeazzi4 aut |
700 | 1 | a Papadimitropoulos, Adamu University Hospital Basel4 aut |
700 | 1 | a Todorov, Atanasu University Hospital Basel4 aut |
700 | 1 | a Epple, Christianu University Hospital Basel4 aut |
700 | 1 | a Peretti, Giuseppe Mu Istituto Ortopedico Galeazzi,University of Milan4 aut |
700 | 1 | a Martin, Ivanu University Hospital Basel4 aut |
710 | 2 | a University Hospital Baselb Molekylär skelettbiologi4 org |
773 | 0 | t International Journal of Molecular Sciencesd : MDPI AGg 21:19q 21:19x 1422-0067 |
856 | 4 | u http://dx.doi.org/10.3390/ijms21197233x freey FULLTEXT |
856 | 4 | u https://www.mdpi.com/1422-0067/21/19/7233/pdf |
856 | 4 8 | u https://lup.lub.lu.se/record/6b8034c4-6268-447d-bd2a-f6e5e313ae29 |
856 | 4 8 | u https://doi.org/10.3390/ijms21197233 |
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