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Estrogenic activity...
Estrogenic activity of surface waters using zebrafish- and human-based in vitro assays : The Danube as a case-study
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- Serra, Hélène (författare)
- Lund University,Lunds universitet,Centrum för miljö- och klimatvetenskap (CEC),Naturvetenskapliga fakulteten,Centre for Environmental and Climate Science (CEC),Faculty of Science,French National Institute for Industrial Environment and Risks,University of Bordeaux
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- Brion, François (författare)
- French National Institute for Industrial Environment and Risks
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- Chardon, Clémence (författare)
- French National Institute for Industrial Environment and Risks
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- Budzinski, Hélène (författare)
- University of Bordeaux
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- Schulze, Tobias (författare)
- Helmholtz Centre for Environmental Research (UFZ)
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- Brack, Werner (författare)
- RWTH Aachen University,Helmholtz Centre for Environmental Research (UFZ)
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- Aït-aïssa, Selim (författare)
- French National Institute for Industrial Environment and Risks
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(creator_code:org_t)
- Elsevier BV, 2020
- 2020
- Engelska 9 s.
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Ingår i: Environmental Toxicology and Pharmacology. - : Elsevier BV. - 1382-6689. ; 78
- Relaterad länk:
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http://dx.doi.org/10...
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https://hal.archives...
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https://lup.lub.lu.s...
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https://doi.org/10.1...
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Abstract
Ämnesord
Stäng
- Most in vitro reporter gene assays used to assess estrogenic contamination are based on human estrogen receptor α (hERα) activation. However, fish bioassays can have distinct response to estrogenic chemicals and mixtures, questioning the relevance of human-based bioassays for assessing risk to this species. In this study, zebrafish liver cells stably expressing zebrafish ERβ2 (ZELHβ2) and human breast cancer cells expressing hERα (MELN) were used to quantify the estrogenic activity of 25 surface water samples of the Danube River, for which chemicals have been previously quantified. Most samples had a low estrogenic activity below 0.1 ng/L 17β-estradiol-equivalents that was more often detected by MELN cells, while ZELHβ2 response tend to be lower than predicted based on the chemicals identified. Nevertheless, both bioassays quantified well a higher estrogenic activity at two sites, which was confirmed in vivo using a transgenic zebrafish assay. The results are discussed considering the effect-based trigger values proposed for water quality monitoring.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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