Desaturation and chain elongation of n - 3 and n - 6 polyunsaturated fatty acids in the human CaCo-2 cell line

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Chen, QLund University,Lunds universitet,Medicin, Lund,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Medicine, Lund,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine (författare)

Desaturation and chain elongation of n - 3 and n - 6 polyunsaturated fatty acids in the human CaCo-2 cell line

Artikel/kapitelEngelska1993

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Elsevier BV,1993
9 s.

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LIBRIS-ID:oai:lup.lub.lu.se:a29a6c22-9502-4217-b218-9e367624afb9
https://lup.lub.lu.se/record/a29a6c22-9502-4217-b218-9e367624afb9URI
https://doi.org/10.1016/0005-2760(93)90097-sDOI

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Språk:engelska
Sammanfattning på:engelska

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Ämneskategori:ref swepub-contenttype

Anmärkningar

Human CaCo-2 cells were incubated with [14C]linoleic (18:2(n - 6)), [14C]linolenic (18:3(n - 3)) and [3H]eicosapentaenoic acid (20:5(n - 3)), and the interconversion of the radioactive fatty acids to higher homologues and their acylation into triacylglycerols (TG) and phospholipids were examined. An active conversion of [14C]18:3 to [14C]20:5 and [14C]docosapentaenoic acid (22:5(n - 3)) and of [3H]20:5 to [3H]22:5, but not to [3H]docosahexaenoic acid (22:6(n - 3)) was observed. In relation to the amounts that had been incorporated into cellular phospholipids and TG, the interconversion of [14C]18:3 clearly exceeded that of [14C]18:2. Addition of 10-100 microM 18:2 or 10-50 microM arachidonic acid (20:4(n - 6)) increased the percent interconversion of [14C]18:2 to [14C]20:4. E.g., addition of 50 microM 20:4 increased the formation of [14C]20:4 from 4.4 +/- 0.1% to 5.9 +/- 0.8%, decreased the incorporation into phospholipids from 64.8 +/- 6.3% to 31.4 +/- 1.2% and increased the incorporation into TG from 8.8 +/- 0.4% to 28.8 +/- 1.1%. In contrast, addition of 10-100 microM 18:3 or 20:5 significantly decreased the interconversion of both [14C]18:2 and [14C]18:3. E.g., addition of 50 microM 20:5 decreased the formation of [14C]20:4 from [14C]18:2 from 4.4 +/- 0.1% to 0.9 +/- 0.1%, whereas the effects on the acylation reactions were very similar to those of 20:4. 20:5 also decreased the formation of interconversion products from [14C]18:3. 18:2 and 20:4 caused a smaller decrease in the formation of [14C]20:5 and actually increased percent conversion to [14C]22:5. The percent conversion of [3H]20:5 to [3H]22:5 was also increased by the addition of 50-100 microM unlabeled 20:5. [14C]18:2 and [14C]18:3 were predominantly incorporated into phosphatidylcholine (PC) whereas more of the radioactive 20:4, 20:5 and 22:5 was incorporated into phosphatidylethanolamine (PE). An active fatty acid interconversion catalyzed by delta 6 and delta 5 desaturases thus occurs in the human CaCo-2 cell line, whereas conversion of 20:5(n - 3) to 22:6(n - 3) could not be demonstrated. The desaturation-elongation pathway has a preference for 18:3(n - 3) and is subjected to an efficient feedback regulation by 20:5(n - 3). Formation of 22:5 increases with available 20:5 mass and by the presence of other polyunsaturated fatty acids competing with 20:5 for acylation into phospholipids.

Ämnesord och genrebeteckningar

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Andra medicinska och farmaceutiska grundvetenskaper hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Other Basic Medicine hsv//eng
Carbon Radioisotopes
Cell Line/metabolism
Fatty Acid Desaturases/metabolism
Fatty Acids, Omega-3/metabolism
Fatty Acids, Omega-6
Fatty Acids, Unsaturated/metabolism
Humans
Phospholipids/metabolism
Time Factors
Triglycerides/metabolism

Biuppslag (personer, institutioner, konferenser, titlar ...)

Nilsson, ALund University,Lunds universitet,Medicin, Lund,Sektion II,Institutionen för kliniska vetenskaper, Lund,Medicinska fakulteten,Gastro,Forskargrupper vid Lunds universitet,Medicine, Lund,Section II,Department of Clinical Sciences, Lund,Faculty of Medicine,Lund University Research Groups(Swepub:lu)med-ani (författare)
Medicin, LundSektion II (creator_code:org_t)

Sammanhörande titlar

Ingår i:Biochimica et Biophysica Acta: Elsevier BV1166:2-3, s. 193-2010006-3002
Ingår i:Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism: Elsevier BV1166:2-3, s. 193-2010005-2760

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Av författaren/redakt...: Chen, Q; Nilsson, A

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