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Comparison of serum...
Comparison of serum testosterone and estradiol measurements in 3174 European men using platform immunoassay and mass spectrometry; relevance for the diagnostics in aging men
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Huhtaniemi, Ilpo T. (author)
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Tajar, Abdelouahid (author)
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Lee, David M. (author)
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O'Neill, Terence W. (author)
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Finn, Joseph D. (author)
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Bartfai, Gyoergy (author)
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Boonen, Steven (author)
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Casanueva, Felipe F. (author)
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- Giwercman, Aleksander (author)
- Lund University,Lunds universitet,Reproduktionsmedicin, Malmö,Forskargrupper vid Lunds universitet,Reproductive medicine, Malmö,Lund University Research Groups
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Han, Thang S. (author)
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Kula, Krzysztof (author)
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Labrie, Fernand (author)
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Lean, Michael E. J. (author)
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Pendleton, Neil (author)
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Punab, Margus (author)
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Silman, Alan J. (author)
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Vanderschueren, Dirk (author)
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Forti, Gianni (author)
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Wu, Frederick C. W. (author)
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(creator_code:org_t)
- 2012
- 2012
- English.
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In: European Journal of Endocrinology. - 1479-683X. ; 166:6, s. 983-991
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http://dx.doi.org/10...
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Abstract
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- Background: The limitations of serum testosterone and estradiol (E-2) measurements using nonextraction platform immunoassays (IAs) are widely recognized. Switching to more specific mass spectrometry (MS)-based methods has been advocated, but directly comparative data on the two methods are scarce. Methods: We compared serum testosterone and E-2 measurements in a large sample of middle-aged/elderly men using a common platform IA and a gas chromatography (GC)-MS method, in order to assess their limitations and advantages, and to diagnose male hypogonadism. Of subjects from the European Male Aging Study (n = 3174; age 40-79 years), peripheral serum testosterone and E-2 were analyzed using established commercial platform IAs (Roche Diagnostics E170) and in-house GC MS methods. Results: Over a broad concentration range, serum testosterone concentration measured by IA and MS showed high correlation (R=0.93, P<0.001), which was less robust in the hypogonadal range (<11 nmol/l; R=0.72, P<0.001). The IA/MS correlation was weaker in E-2 measurements (R=0.32, P<0.001, at E-2 <40.8 pmol/l, and R=0.74, P<0.001, at E-2 >40.8 pmol/l). Using MS as the comparator method, IA ascertained low testosterone compatible with hypogonadism (<11 nmol/l), with 75% sensitivity and 96.3% specificity. The same parameters with IA for the detection of low E-2 (<40.7 pmol/l) were 13.3 and 99.3%, and for high E-2 (>120 pmol/l) 88.4 and 88.6%. Conclusion: A validated platform IA is sufficient to detect subnormal testosterone concentrations in the diagnosis of male hypogonadism. The IA used for E-2 measurements showed poor correlation with MS and may only be suitable for the detection of high E-2 in men.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Publication and Content Type
- art (subject category)
- ref (subject category)
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- By the author/editor
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Huhtaniemi, Ilpo ...
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Tajar, Abdelouah ...
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Lee, David M.
-
O'Neill, Terence ...
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Finn, Joseph D.
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Bartfai, Gyoergy
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show more...
-
Boonen, Steven
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Casanueva, Felip ...
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Giwercman, Aleks ...
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Han, Thang S.
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Kula, Krzysztof
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Labrie, Fernand
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Lean, Michael E. ...
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Pendleton, Neil
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Punab, Margus
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Silman, Alan J.
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Vanderschueren, ...
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Forti, Gianni
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Wu, Frederick C. ...
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show less...
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Endocrinology an ...
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European Journal ...
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Lund University