Sökning: onr:"swepub:oai:lup.lub.lu.se:be2d508d-75ca-4fb3-afac-69783e99ad25" >
Identification of t...
Identification of the ligand of Pru p 3, a peach LTP
-
- Cubells-Baeza, Nuria (författare)
- Technical University of Madrid,Centre for Plant Biotechnology and Genomics
-
- Gómez-Casado, Cristina (författare)
- Lund University,Lunds universitet,Slemhinnans immunologi,Forskargrupper vid Lunds universitet,Mucosal Immunology,Lund University Research Groups
-
- Tordesillas, Leticia (författare)
- Icahn School of Medicine at Mount Sinai
-
visa fler...
-
- Ramírez-Castillejo, Carmen (författare)
- Centre for Plant Biotechnology and Genomics,Technical University of Madrid
-
- Garrido-Arandia, María (författare)
- Centre for Plant Biotechnology and Genomics,Technical University of Madrid
-
- González-Melendi, Pablo (författare)
- Technical University of Madrid
-
- Herrero, María (författare)
- CSIC - Estacion Experimental de Aula Dei EEAD
-
- Pacios, Luis F. (författare)
- Centre for Plant Biotechnology and Genomics,Technical University of Madrid
-
- Díaz-Perales, Araceli (författare)
- Centre for Plant Biotechnology and Genomics,Technical University of Madrid
-
visa färre...
-
(creator_code:org_t)
- 2017-03-15
- 2017
- Engelska 12 s.
-
Ingår i: Plant Molecular Biology. - : Springer Science and Business Media LLC. - 0167-4412 .- 1573-5028. ; 94:1-2, s. 33-44
- Relaterad länk:
-
http://dx.doi.org/10...
-
visa fler...
-
http://oa.upm.es/500...
-
https://lup.lub.lu.s...
-
https://doi.org/10.1...
-
visa färre...
Abstract
Ämnesord
Stäng
- Key message: Pru p 3, a peach LTP, is located in pollinated flower styles and secreting downy hairs, transporting a derivative of camptothecin bound to phytosphingosine. Pru p 3 may inhibit a second pollination and may keep away herbivores until seed maturation. Abstract: The allergen Pru p 3, a peach lipid transfer protein, has been well studied. However, its physiological function remains to be elucidated. Our results showed that Pru p 3 usually carries a lipid ligand that play an essential role in its function in plants. Using ESI-qToF, we observed that the ligand was a derivative of camptothecin binding to phytosphingosine, wich that is inserted into the hydrophobic tunnel of the protein. In addition, the described ligand displayed topoisomerase I activity inhibition and self-fluorescence, both recognized as camptothecin properties. During flower development, the highest expression of Pru p 3 was detected in the styles of pollinated flowers, in contrast to its non-expression in unpollinated pistils, where expression decreased after anthesis. During ripening, the expression of Pru p 3 were observed mainly in peel but not in pulp. In this sense, Pru p 3 protein was also localized in trichomes covering the fruit epidermis.
Ämnesord
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Biologi -- Botanik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Botany (hsv//eng)
Nyckelord
- Camptothecin
- Flower development
- Fruit development
- Lipid transfer protein
- Pollination
- Pru p 3
- Secondary metabolites
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
Hitta via bibliotek
Till lärosätets databas