Proteomics profiling of human synovial fluid suggests increased protein interplay in early-osteoarthritis (OA) that is lost in late-stage OA

Ali, NeserinLund University,Lunds universitet,Lund OsteoArthritis Division - Clinical Epidemiology Unit,Forskargrupper vid Lunds universitet,Lund University Research Groups (author)

The underlying molecular mechanisms in osteoarthritis (OA) development are largely unknown. This study explores the proteome and the pairwise interplay of proteins in synovial fluid from patients with late-stage knee OA (arthroplasty), early knee OA (arthroscopy due to degenerative meniscal tear) and from deceased controls without knee OA.Synovial fluid samples were analyzed using state-of-the-art mass spectrometry with data-independent acquisition. The differential expression of the proteins detected was clustered and evaluated with data mining strategies and a multilevel model. Group-specific slopes of associations were estimated between expressions of each pair of identified proteins to assess the co-expression (i.e. interplay) between the proteins in each group.More proteins were increased in early-OA vs controls than late-stage OA vs controls. For most of these proteins, the fold changes between late-stage OA vs controls and early stage OA vs controls were remarkably similar suggesting potential involvement in the OA process. Further, for the first time this study illustrated distinct patterns in protein co-expression suggesting that the interplay between the protein machinery is increased in early-OA and lost in late-stage OA. Further efforts should focus on earlier stages of the disease than previously considered.

Turkiewicz, AleksandraLund University,Lunds universitet,Lund OsteoArthritis Division - Clinical Epidemiology Unit,Forskargrupper vid Lunds universitet,Lund University Research Groups(Swepub:lu)med-ati (author)
Hughes, VelocityLund University,Lunds universitet,Lund OsteoArthritis Division - Clinical Epidemiology Unit,Forskargrupper vid Lunds universitet,Lund University Research Groups(Swepub:lu)ha0258ve (author)
Folkesson, ElinLund University (author)
Tjörnstand, JonLund University,Lunds universitet,Forskningsgruppen för höft- och knäproteskirurgi,Forskargrupper vid Lunds universitet,The Hip and Knee Joint Arthroplasty Research Group,Lund University Research Groups,Skåne University Hospital(Swepub:lu)orto-jtj (author)
Neuman, PaulSkåne University Hospital(Swepub:lu)orto-pne (author)
Önnerfjord, PatrikLund University,Lunds universitet,Molekylär skelettbiologi,Forskargrupper vid Lunds universitet,Molecular Skeletal Biology,Lund University Research Groups(Swepub:lu)akem-pon (author)
Englund, MartinLund University,Lunds universitet,Lund OsteoArthritis Division - Clinical Epidemiology Unit,Forskargrupper vid Lunds universitet,Lund University Research Groups(Swepub:lu)ort-med (author)
Lund OsteoArthritis Division - Clinical Epidemiology UnitForskargrupper vid Lunds universitet (creator_code:org_t)

By the author/editor: Ali, Neserin; Turkiewicz, Alek ...; Hughes, Velocity; Folkesson, Elin; Tjörnstand, Jon; Neuman, Paul; show more...; Önnerfjord, Patr ...; Englund, Martin; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Rheumatology and ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Orthopaedics

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