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Summary report of t...
Abstract
Ämnesord
Stäng
- Twelve research groups participated in the ISOBM TD-3 Workshop in which the reactivity and specificity of 83 antibodies against prostate-specific antigen (PSA) were investigated. Using a variety of techniques including cross-inhibition assays, Western blotting, BIAcore, immunoradiometric assays and immunohistochemistry, the antibodies were categorized into six major groups which formed the basis for mapping onto two- and three-dimensional (2-D and 3-D) models of PSA. The overall findings of the TD-3 Workshop are summarized in this report. In agreement with all participating groups, three main antigenic domains were identified: free PSA-specific epitopes located in or close to amino acids 86-91; discontinuous epitopes specific for PSA without human kallikrein (hK2) cross-reactivity located at or close to amino acids 158-163; and continuous or linear epitopes shared between PSA and hK2 located close to amino acids 3-11. In addition, several minor and partly overlapping domains were also identified. Clearly, the characterization of antibodies from this workshop and the location of their epitopes on the 3-D model of PSA illustrate the importance of selecting appropriate antibody pairs for use in immunoassays. It is hoped that these findings and the epitope nomenclature described in this TD-3 Workshop are used as a standard for future evaluation of anti-PSA antibodies.
Ämnesord
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Nyckelord
- PSA
- Prostate
- Antibody
- Workshop
- ISOBM
Publikations- och innehållstyp
- art (ämneskategori)
- ref (ämneskategori)
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Till lärosätets databas
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Stenman, U H
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Paus, E
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Allard, W J
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Andersson, I
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Andres, C
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Barnett, T R
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Becker, Charlott ...
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Belenky, A
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Bellanger, L
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Pellegrino, C M
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Bormer, O P
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Davis, G
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Dowell, B
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Grauer, L S
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Jette, D C
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Karlsson, B
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Kreutz, F T
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van der Kwast, T ...
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Lauren, L
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Leinimaa, M
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Leinonen, J
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Lilja, Hans
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Linton, H J
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Nap, M
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Nilsson, O
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Ng, P C
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Nustad, K
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Peter, A
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Pettersson, K
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Piironen, T
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Rapp, J
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Rittenhouse, H G
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Rye, P D
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Seguin, P
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Slota, J
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Sokoloff, R L
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Suresh, M R
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Very, D L
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Wang, T J
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Wigheden, Ingrid
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Wolfert, R L
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Yeung, K K
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Zhang, W-M
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Zhou, Z
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Hilgers, J
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