Sökning: onr:"swepub:oai:openarchive.ki.se:10616/48411" > Amelioration of sys...
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001 | oai:openarchive.ki.se:10616/48411 | |
003 | SwePub | |
008 | 240910s2021 | |||||||||||000 ||eng| | |
009 | oai:prod.swepub.kib.ki.se:147830459 | |
022 | a 2157-846X | |
024 | 7 | a 10616/484112 hdl |
024 | 7 | a http://hdl.handle.net/10616/484112 URI |
024 | 7 | a https://doi.org/10.1038/s41551-021-00792-z2 DOI |
024 | 7 | a http://kipublications.ki.se/Default.aspx?queryparsed=id:1478304592 URI |
040 | a (SwePub)ki | |
041 | a engb eng | |
042 | 9 SwePub | |
072 | 7 | a ref2 swepub-contenttype |
072 | 7 | a art2 swepub-publicationtype |
100 | 1 | a Gupta, Dhanuu Karolinska Institutet4 aut |
245 | 1 0 | a Amelioration of systemic inflammation via the display of two different decoy protein receptors on extracellular vesicles |
264 | c 2021-10-06 | |
264 | 1 | a Stockholm :b Karolinska Institutet, Dept of Laboratory Medicine,c 2021 |
338 | a electronic2 rdacarrier | |
520 | a Extracellular vesicles (EVs) can be functionalized to display specific protein receptors on their surface. However, surface-display technology typically labels only a small fraction of the EV population. Here, we show that the joint display of two different therapeutically relevant protein receptors on EVs can be optimized by systematically screening EV-loading protein moieties. We used cytokine-binding domains derived from tumour necrosis factor receptor 1 (TNFR1) and interleukin-6 signal transducer (IL-6ST), which can act as decoy receptors for the pro-inflammatory cytokines tumour necrosis factor alpha (TNF-α) and IL-6, respectively. We found that the genetic engineering of EV-producing cells to express oligomerized exosomal sorting domains and the N-terminal fragment of syntenin (a cytosolic adaptor of the single transmembrane domain protein syndecan) increased the display efficiency and inhibitory activity of TNFR1 and IL-6ST and facilitated their joint display on EVs. In mouse models of systemic inflammation, neuroinflammation and intestinal inflammation, EVs displaying the cytokine decoys ameliorated the disease phenotypes with higher efficacy as compared with clinically approved biopharmaceutical agents targeting the TNF-α and IL-6 pathways. | |
700 | 1 | a Wiklander, Oscar P Bu Karolinska Institutet4 aut |
700 | 1 | a Görgens, Andréu Karolinska Institutet4 aut |
700 | 1 | a Conceição, Mariana4 aut |
700 | 1 | a Corso, Giuliau Karolinska Institutet4 aut |
700 | 1 | a Liang, Xiumingu Karolinska Institutet4 aut |
700 | 1 | a Seow, Yiqi4 aut |
700 | 1 | a Balusu, Sriram4 aut |
700 | 1 | a Feldin, Ulrika4 aut |
700 | 1 | a Bostancioglu, Beklem4 aut |
700 | 1 | a Jawad, Rimu Karolinska Institutet4 aut |
700 | 1 | a Mamand, Doste Ru Karolinska Institutet4 aut |
700 | 1 | a Lee, Yi Xin Fiona4 aut |
700 | 1 | a Hean, Justin4 aut |
700 | 1 | a Mäger, Imre4 aut |
700 | 1 | a Roberts, Thomas C4 aut |
700 | 1 | a Gustafsson, Manuelau Karolinska Institutet4 aut |
700 | 1 | a Mohammad, Dara Ku Karolinska Institutet4 aut |
700 | 1 | a Sork, Helenau Karolinska Institutet4 aut |
700 | 1 | a Backlund, Alexandra4 aut |
700 | 1 | a Lundin, Per4 aut |
700 | 1 | a de Fougerolles, Antonin4 aut |
700 | 1 | a Smith, C I Edvardu Karolinska Institutet4 aut |
700 | 1 | a Wood, Matthew J A4 aut |
700 | 1 | a Vandenbroucke, Roosmarijn E4 aut |
700 | 1 | a Nordin, Joel Zu Karolinska Institutet4 aut |
700 | 1 | a El-Andaloussi, Samiru Karolinska Institutet4 aut |
710 | 2 | a Karolinska Institutet |
710 | 2 | a Karolinska Institutet |
710 | 2 | a Karolinska Institutet4 org |
773 | 0 | t Nature Biomedical Engineeringd Stockholm : Karolinska Institutet, Dept of Laboratory Medicinex 2157-846X |
856 | 4 | u http://hdl.handle.net/10616/48411x primaryx Object in contextx freey FULLTEXT |
856 | 4 8 | u http://hdl.handle.net/10616/48411 |
856 | 4 8 | u https://doi.org/10.1038/s41551-021-00792-z |
856 | 4 8 | u http://kipublications.ki.se/Default.aspx?queryparsed=id:147830459 |
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