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A Circulating Micro...
A Circulating MicroRNA Profile in a Laser-Induced Mouse Model of Choroidal Neovascularization
- Artikel/kapitelEngelska2020
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LIBRIS-ID:oai:prod.swepub.kib.ki.se:143890568
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http://kipublications.ki.se/Default.aspx?queryparsed=id:143890568URI
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https://doi.org/10.3390/ijms21082689DOI
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Språk:engelska
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Sammanfattning på:engelska
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Ämneskategori:art swepub-publicationtype
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Choroidal neovascularization (CNV) is a pathological process in which aberrant blood vessels invade the subretinal space of the mammalian eye. It is a characteristic feature of the prevalent neovascular age-related macular degeneration (nAMD). Circulating microRNAs (cmiRNAs) are regarded as potentially valuable biomarkers for various age-related diseases, including nAMD. Here, we investigated cmiRNA expression in an established laser-induced CNV mouse model. Upon CNV induction in C57Bl/6 mice, blood-derived cmiRNAs were initially determined globally by RNA next generation sequencing, and the most strongly dysregulated cmiRNAs were independently replicated by quantitative reverse transcription PCR (RT-qPCR) in blood, retinal, and retinal pigment epithelium (RPE)/choroidal tissue. Our findings suggest that two miRNAs, mmu-mir-486a-5p and mmur-mir-92a-3p, are consistently dysregulated during CNV formation. Furthermore, in functional in vitro assays, a significant impact of mmu-mir-486a-5p and mmu-mir-92a-3p on murine microglial cell viability was observed, while mmu-mir-92a-3p also showed an impact on microglial mobility. Taken together, we report a robust dysregulation of two miRNAs in blood and RPE/choroid after laser-induced initiation of CNV lesions in mice, highlighting their potential role in pathology and eventual therapy of CNV-associated complications.
Biuppslag (personer, institutioner, konferenser, titlar ...)
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Berber, P
(författare)
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Karlstetter, M
(författare)
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Aslanidis, A
(författare)
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Strunz, T
(författare)
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Langmann, T
(författare)
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Grassmann, FKarolinska Institutet
(författare)
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Weber, BHF
(författare)
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Karolinska Institutet
(creator_code:org_t)
Sammanhörande titlar
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Ingår i:International journal of molecular sciences: MDPI AG21:81422-0067
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