D-Lactate dehydrogenase as a marker gene allows positive selection of transgenic plants

Wienstroer, J. (författare): Heinrich Heine Universität Düsseldorf,Heinrich Heine University Düsseldorf

Engqvist, Martin, 1983 (författare): Universität zu Köln,University of Cologne,California Institute of Technology (Caltech)

Maurino, V.G. (författare): Heinrich Heine Universität Düsseldorf,Heinrich Heine University Düsseldorf,Universität zu Köln,University of Cologne

(creator_code:org_t)

2011-12-08
2012
Engelska.
Ingår i: FEBS Letters. - : Wiley. - 1873-3468 .- 0014-5793. ; 586:1, s. 36-40

Relaterad länk:: http://dx.doi.org/10...; visa fler...; https://research.cha...; https://doi.org/10.1...; visa färre...

Abstract Ämnesord

Stäng

d-Lactate negatively affects Arabidopsis thaliana seedling development in a concentration-dependent manner. At media d-lactate concentrations greater than 5-10 mM the development of wild-type plants is arrested shortly after germination whereas plants overexpressing the endogenous d-lactate dehydrogenase (d-LDH) detoxify d-lactate to pyruvate and survive. When the transgenic plants are further transferred to normal growth conditions they develop indistinguishably from the wild type. Thus, d-LDH was successfully established as a new marker in A. thaliana allowing selecting transgenic plants shortly after germination. The selection on d-lactate containing media adds a new optional marker system, which is especially useful if the simultaneous selection of multiple constructs is desired.

Av författaren/redakt...: Wienstroer, J.; Engqvist, Martin ...; Kunz, H.H.; Flügge, U.I.; Maurino, V.G.

Om ämnet

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