Human fibroblast and human bone marrow cell response to lithographically nanopatterned adhesive domains on protein rejecting substrates.

• The separate influence of topographical and chemical cues on cell attachment and spreading are well documented; however, that of duel-cue substrates is less so. In this study graft copolymers that sterically stabilize biological surfaces were employed alongside nanotopographical features fabricated by colloidal lithography. This resulted in the production of a range of substrates whereby the effect of chemistry and or topography on both on human fibroblast and bone marrow cell adhesion and spreading could be observed. The current studies indicate an enhancement of cell response as a consequence of modifications in material topography, whereas the current selected chemical cues inhibited cell function. Critically, in combination, topography modulated the effects of chemical environment.

Keyword

methods

Cell Proliferation

Cell Culture Techniques

Cells

chemistry

Tissue Engineering

Surface Properties

chemistry

methods

metabolism

Humans

metabolism

Cultured

Bone Marrow Cells

Nanostructures

Materials Testing

cytology

Cell Survival

Fibroblasts

cytology

Cell Adhesion

metabolism

physiology

Biocompatible Materials

chemistry

ultrastructure

Substrate Specificity

Proteins

Publication and Content Type

art (subject category)

ref (subject category)